We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Trypsin/Lys-C Mix, Improved Proteolytic Efficiency for Enhanced Reproducible Results

Need Assistance? Chat

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and Lys-C, Mass Spec Grade. Trypsin/Lys-C Mix is recommended for use in two applications, enhanced standard in-solution protein digestion and in-solution digestion of proteolytically resistant proteins.

Download Video:
Closed Format: MP4
Open Format: OGG

Enhanced Proteolysis

Using standard digestion conditions, Trypsin/Lys-C Mix misses fewer cleavage sites than Trypsin alone (Figure 1). This improved digestion increases peptide recovery and results in better protein quantitation (Figure 2) as well as improved reproducibility (Figure 3).

Figure 1. Side-by-side comparison of cleavage sites missed by trypsin or the Trypsin/Lys-C Mix using a standard digestion protocol.
/~/media/images/resources/figures/11700-11799/11788mb_800px.png
Figure 2. The Trypsin/Lys-C Mix provides a critical advantage for protein quantitation. Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.
/~/media/images/resources/figures/11700-11799/11787ma_800px.png
Figure 3. Increase the number of identified proteins/reproducibility. Digestion of a HeLa extract with Trypsin/Lys-C Mix increases the number of identified peptides/proteins and improves reproducibility when compared to standard trypsin digestion.
/~/media/images/resources/figures/11300-11399/11380ma_800px.png

Tolerance for Trypsin-inhibiting Contaminants

Use the Trypsin/Lys-C Mix to generate mass spectrometry data from poor-quality sample material that contains reagents that inhibit trypsin activity (i.e., FFPE tissue and plant samples).

Figure 4. Improved mass spec analysis of a poorly purified protein extract. The extract contained residual amount of methanol and chloroform, which inhibits trypsin, but not Trypsin/Lys-C Mix.

/~/media/images/resources/figures/11200-11299/11221ma_750px.png

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.