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Promega Corporation

Trypsin/Lys-C Mix, Improved Proteolytic Efficiency for Enhanced Reproducible Results

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Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and Lys-C, Mass Spec Grade. Trypsin/Lys-C Mix is recommended for use in two applications, enhanced standard in-solution protein digestion and in-solution digestion of proteolytically resistant proteins.

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Enhanced Proteolysis

Using standard digestion conditions, Trypsin/Lys-C Mix misses fewer cleavage sites than Trypsin alone (Figure 1). This improved digestion increases peptide recovery and results in better protein quantitation (Figure 2) as well as improved reproducibility (Figure 3).

Figure 1. Side-by-side comparison of cleavage sites missed by trypsin or the Trypsin/Lys-C Mix using a standard digestion protocol.
Figure 2. The Trypsin/Lys-C Mix provides a critical advantage for protein quantitation. Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.
Figure 3. Increase the number of identified proteins/reproducibility. Digestion of a HeLa extract with Trypsin/Lys-C Mix increases the number of identified peptides/proteins and improves reproducibility when compared to standard trypsin digestion.

Tolerance for Trypsin-inhibiting Contaminants

Use the Trypsin/Lys-C Mix to generate mass spectrometry data from poor-quality sample material that contains reagents that inhibit trypsin activity (i.e., FFPE tissue and plant samples).

Figure 4. Improved mass spec analysis of a poorly purified protein extract. The extract contained residual amount of methanol and chloroform, which inhibits trypsin, but not Trypsin/Lys-C Mix.


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