- Improve protein sequence coverage by combining digestion with trypsin and an alternative protease.
- Enhance digestion of tightly folded proteins by using proteases that tolerate denaturing conditions.
- Identify post-translational modifications (PTM) that are resistant to trypsin digestion by using an alternative protease.
Increase Protein Sequence Coverage
Digestion with alternative proteases individually or in combination will create a unique peptide map that may include sequences not seen with trypsin digestion alone. Overlaying these peptides with those obtained with trypsin increases protein coverage and overall confidence in protein identification.
Increase Protein Sequence Coverage With:
Enhance Digestion of Difficult Proteins with Thermolysin and Pepsin
Thermolysin and pepsin are unique because they tolerate conditions that can be used to denature difficult to digest and tightly folded proteins, high temperatures and low pH, respectively. Because they remain active under these conditions, these proteases can cleave previously inaccessible sites.
Enhance Digestion of Difficult Proteins With:
Characterize Post-translation Modifications with Arg-C
Some Post-translation Modifications (PTM), such as acetylation and methylation, prevent trypsin digestion. As a result, digesting with trypsin alone can miss certain PTMs. Including digestion with Arg-C can identify more PTMs, including mono-, dimethylated and acetylated lysine and arginine residues.
Improve Data for PTM Analysis With: