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Promega Corporation

Protein-Works

protein-works

Mass spectrometry protein-analysis services encompassing protein identification, protein sequence confirmation, protein molecular weight determination, complex sample profiling and complex sample differential analysis.

PROTEIN IDENTIFICATION

Proteins are digested with trypsin and the resulting peptides are analyzed by LC/MS/MS. The peptides are fragmented in the mass spectrometer to yield diagnostic patterns that can be matched to protein sequence databases via computer algorithms. The sensitivity of this technique is 1ng or less starting material for a given protein.

Solutions containing a simple buffer background (e.g. water or volatile salts) are amenable to direct in-solution digest. More complex backgrounds (e.g. detergent, non-volatile salts, glycerol) require an additional clean-up step to facilitate the sample analysis (MSB-02).

SERVICE ID SERVICE DESCRIPTION LIST PRICE PER SAMPLE
MSB-01 Protein ID in-gel digest $250
MSB-02 Protein ID in-solution digest $450

Sample types: 2D gel spots, SDS-PAGE bands, recombinant proteins, purified proteins
Data turnaround time: Two weeks or less
Report format: PDF report

PROTEIN MAPPING

This service confirms the N-terminal to C-terminal sequence of a protein. The analytical strategy involves using multiple enzymes of differing specificities—trypsin, chymotrypsin and pepsin—with LC/MS/MS. The peptides are fragmented in the mass spectrometer to yield diagnostic patterns that can be matched to protein databases via computer algorithms. Data from all experiments are compiled with the multiple specificities of the enzymes allowing for complete protein sequence coverage.

SERVICE ID SERVICE DESCRIPTION LIST PRICE PER SAMPLE
MSB-03 Protein Mapping $800

Sample types: 2D gel spots or SDS-PAGE bands, recombinant proteins, purified proteins
Data turnaround time: Two weeks or less
Report format: PDF report

INTACT MASS MEASUREMENT

This service enables the determination of the molecular weight of a protein. High-purity samples are required.

SERVICE ID SERVICE DESCRIPTION LIST PRICE PER SAMPLE
MSB-04 Intact Mass Measurement $390

Sample types: Recombinant proteins, purified proteins
Data turnaround time: Two weeks or less
Report format: PDF report

PROTEIN PROFILING

This service provides a qualitative profile of proteins in a complex sample such as a cell lysate, a tissue homogenate or a biofluid. The extent of fractionation performed on the sample prior to the analysis dictates the number of proteins observed. The greater the number of fractions, the greater the number of proteins identified and sequence coverage of those proteins.

Sample or fractions are digested using trypsin, and the resulting peptide mixture is analyzed by LC/MS/MS. The peptides are fragmented in the mass spectrometer to yield diagnostic patterns that can be matched to protein sequence databases via computer algorithms. A non-redundant list of proteins present in the sample is determined with associated pathway information.

A 10-fraction analysis is suitable for the analysis of low-complexity samples e.g. HPLC fractions, semi-purified extracts. A 20-fraction analysis is for medium-complexity samples e.g., subcellular fractions, pooled HPLC fractions. A 40-fraction analysis is suitable for complex samples e.g., whole cell lysates, whole tissue extracts.

SERVICE ID SERVICE DESCRIPTION LIST PRICE PER SAMPLE
MSB-05 Protein profiling unfractionated $650
MSB-06 Protein profiling 10 fractions $1,450
MSB-07 Protein profiling 20 fractions $2,100
MSB-08 Protein profiling 40 fractions $3,500

Sample types: Lysates, cells, tissues, biofluids, FFPE protein extracts
Data turn around: Two weeks or less
Report format: PDF report

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