IP-works services are designed for the analysis of immunoprecipitated or co-immunoprecipitated proteins. These experiments can be used to simply profile the protein content of a sample or for the quantitative comparison of control versus test samples e.g., discriminate specific from non-specific binding events.
Samples are fractionated prior to digestion with trypsin, and the resulting peptides are analyzed by LC/MS/MS followed by database searching. A non-redundant list of proteins present in the sample is determined with their associated pathway information. When two or more samples are being compared, a quantitative measure for each protein is provided.
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Sample types: IP'd or co-IP'd proteins/protein complexes, isolated protein fractions.
Data turnaround time: Two weeks or less
Report format: PDF report