Determine Total, GSSG and GSH/GSSG ratios
A549 lung carcinoma cells (5,000 cells/well) were exposed for 60 minutes to menadione doses in Hank’s Balanced Salt Solution.
GSH/GSSG ratios in various cell lines
Cells were treated with 40μM menadione or vehicle directly in tissue culture wells. The ratio was calculated directly from RLUs or after conversion of RLUs to micromoles glutathione using the GSH standard curve.
See protocol for calculations