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Promega Corporation

Promega GoTaq® G2 Polymerase at a Glance

GoTaq® G2 DNA Polymerase is the second generation of GoTaq® products and provides reliable amplification of a wide range of PCR templates, with improved manufacturing processes, increased reliability and consistency. The enzyme is available in many formats to give you maximum flexibility, control and convenience for your PCR.

  • Choosing a Kit
  • Hot Start Master Mix
  • Hot Start Polymerase
  • DNA Polymerases
  • Master Mix
  Hot Start PCR Routine PCR
  GoTaq® G2 Hot Start Green and Colorless Master Mixes GoTaq® G2 Hot Start DNA Polymerase GoTaq® G2 Flexi DNA Polymerase GoTaq® G2 DNA Polymerase GoTaq® G2 Green and Colorless Master Mixes
Specificity Plus Plus Plus Plus Plus Plus Plus Plus Plus
Sensitivity Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus
Yield Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus Plus
Maximum Convenience Plus Plus Plus Plus Plus Plus Plus Plus Plus
Direct-to-Gel Loading (with Green Buffer) checkMark checkMark checkMark checkMark checkMark
Room-Temperature Setup checkMark checkMark
Magnesium Flexibility checkMark checkMark
5´→3´ Exonuclease Activity checkMark checkMark checkMark checkMark checkMark
Activation Time 2 minutes 2 minutes Immediate Immediate Immediate
Amplicon Size up to 5kb up to 5kb up to 5kb up to 5kb up to 5kb
  Ordering Information Ordering Information Ordering Information Ordering Information Ordering Information

Enjoy the convenience of room-temperature setup in a ready-to-use master mix format

  • Simplify reaction setup and save time with a ready-to-use master mix
  • Prepare your reaction at room temperature, not on ice
  • Eliminate nonspecific amplification with hot start enzyme
  • Choose from green or colorless options
  • Use at no risk: Backed by the Promega PCR Satisfaction Guarantee

Combined specificity and yield with direct-to-gel loading convenience

Figure 11416TB

GoTaq® G2 Hot Start Master Mix displays superior sensitivity and robust yield. The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using the GoTaq® G2 Hot Start Green (G) and Colorless (C) Master Mix and the leading competitors' products (L1, L2, Q+ and T) according to manufacturers’ protocols.

Enjoy the convenience of room-temperature setup and higher performance than standard Taq

  • Prepare your reaction at room temperature, not on ice
  • Eliminate nonspecific amplification with hot start enzyme
  • Optimize reaction conditions with a Flexi Reaction Buffer and separate MgCl2
  • Use at no risk: Backed by the Promega PCR Satisfaction Guarantee

Detect lower-copy-number targets

Figure 11414TB

GoTaq® G2 Hot Start Polymerase exhibits higher yields and greater detection sensitivity than competitors'. The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using the GoTaq® G2 Hot Start Polymerase with Green (G) and Colorless (C) Buffers and the leading competitors' products (L1, R, Q, Q+, L2 and L3) according to manufacturers’ recommendations.

Robust, Reliable Amplification

  • Direct-to-gel amplification buffer
  • Two buffer systems available to match your needs
    • 5X Reaction Buffer with MgCl2 to simplify reaction setup
    • 5X Flexi Buffer and separate MgCl2 to enable optimization
  • Risk-free: Backed by the Promega PCR Satisfaction Guarantee

Consistent, robust amplification across different targets

Figure 11408TC

GoTaq® G2 DNA Polymerase consistently produces high yields across multiple targets, while competitors produce highly variable results. Three different targets were amplified with the GoTaq® G2 DNA Polymerase Green (G) and Colorless (C) buffers or competitors' products (T, Q, L1, L2 and R) according to manufacturers’ recommended protocols using 3.3ng of input human DNA.

For maximum convenience of an all-in-one master mix.

  • Simplify reaction setup and save time with a ready-to-use master mix
  • Choose from green or colorless options
  • Add template and primers, and go
  • Use at no risk: Backed by the Promega PCR Satisfaction Guarantee

Consistent, robust amplification across different targets

Figure 11408TB-b

GoTaq® G2 DNA Polymerase Master Mixes consistently produce high yields across multiple targets, while competitors' products highly variable results. Three different targets were amplified with the GoTaq® G2 DNA Polymerase Green (G) and Colorless (C) Master mixes or competitors' products (T, Q, L1, L2 and R) according to manufacturers’ recommended protocols using 3.3ng of input human DNA.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

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