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Promega Corporation

FuGENE® Transfection Reagents

  • FuGENE® 6 Transfection Reagent
  • FuGENE® HD Transfection Reagent
  • Proven Performance: Use the technology published in 1000s of citations
  • More Biological Relevance: Low toxicity, less impact on biology
  • Simple Protocol: No medium changes, less variability, compatible with serum
  • Trusted Promega Quality: Backed by global Technical Support

Use FuGENE® 6 Transfection Reagent with many common cell lines as cited in over 10,000 publications .

Use FuGENE® HD Transfection Reagent for ultimate performance in standard and difficult-to-transfect cell lines .

FuGENE® 6 Transfection Reagent provides robust results in a wide variety of standard laboratory cell lines, making it ideal for everyday use. The simple protocol eliminates tedious culture medium changes and eliminates a source of variation. The gentle performance of the FuGENE® 6 Transfection Reagent makes it an ideal companion to luciferase reporter gene assays or other sensitive cellular analysis applications.


Overview of adherent cell transfection protocol for a 96-well plate


Comparison of transfection reagent performance in HEK293 Cells. Cells were transfected with a luciferase reporter plasmid, and luciferase activity was detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. The FuGENE®6 Transfection Reagent provides high expression levels with minimal impact on cell health.


FuGENE is a registered trademark of Fugent, LLC, USA. *You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

See terms of use at www.promega.com/lull

FuGENE is a registered trademark of Fugent, LLC. Lipofectamine is a trademark of Life Technologies , Inc. and TransIT is a registered trademark of Mirus Bio, LLC

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Scientists at Your Service

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