FuGENE® Transfection Reagent

FuGENE 6 Transfection Reagent provides robust results in a wide variety of standard laboratory cell lines making it ideal for everyday use. The simple protocol eliminates tedious culture media changes and eliminates a source of variation. The gentle performance of FuGENE 6 makes it an ideal companion to luciferase reporter gene assays or other sensitive cellular analysis applications.

Overview of adherent cell transfection protocol for a 96-well plate

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Comparison of Transfection Reagent Performance in HEK293 Cells. Cells were transfected with a luciferase reporter plasmid and detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. FuGENE™ 6 provides high expression levels with minimal impact on cell health.

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FuGENE is a registered trademark of Fugent, LLC, USA. *You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

NOTICE TO PURCHASER: LIMITED LICENSE This product is sold under license from Life Technologies Corporation, Carlsbad, CA. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product solely for research use only. The buyer cannot use this product or its components for any clinical, therapeutic, diagnostic, vaccine, protective, or other uses. The buyer cannot sell or resell this product, whether or not this product is sold or resold for use in research. For information about purchasing a license to use this product or the technology embedded in it for any purpose other than research, contact Outlicensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008. Phone (760) 603-7200. e-mail: outlicensing@lifetech.com

FuGENE is a registered trademark of Fugent, LLC. Lipofectamine is a trademark of Life Technologies , Inc. and TransIT is a registered trademark of Mirus Bio, LLC

FuGENE HD offers unrivaled transfection performance with minimal impact on cell physiology (Jacobson et al; Biotechniques 47 (1) p617-24. 2009). It is ideal for use in a variety of challenging applications including transfection of cancer model cell lines, insect cells, stem cells, and virus and protein production. FuGENE HD allows for more biologically relevant results by allowing you to use the best cell line for your study and doesn't require harsh treatment of the cells by lowering serum concentration or washing of toxic lipid complexes. The in vivo environment can even be mimicked with transfection in100% serum

Comparison of Transfection Reagent Performance in HEK293 Cells. Cells were transfected with a luciferase reporter plasmid and detected using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay. FuGENE™ HD provides high expression levels with minimal impact on cell health.

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Overview of adherent cell transfection protocol for a 96-well plate

/~/media/Images/Resources/Figures/8600-8699/8611TB-425.ashx?w=425&h=636&as=1

FuGENE is a registered trademark of Fugent, LLC, USA. *You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

NOTICE TO PURCHASER: LIMITED LICENSE This product is sold under license from Life Technologies Corporation, Carlsbad, CA. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product solely for research use only. The buyer cannot use this product or its components for any clinical, therapeutic, diagnostic, vaccine, protective, or other uses. The buyer cannot sell or resell this product, whether or not this product is sold or resold for use in research. For information about purchasing a license to use this product or the technology embedded in it for any purpose other than research, contact Outlicensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008. Phone (760) 603-7200. e-mail: outlicensing@lifetech.com

FuGENE is a registered trademark of Fugent, LLC. Lipofectamine is a trademark of Life Technologies , Inc. and TransIT is a registered trademark of Mirus Bio, LLC