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Promega Corporation

QuantiFluor® ssDNA System

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Highly sensitive, easy to use, fluorescent dye for ssDNA Quantitation

QuantiFluor® ssDNA System

OVERVIEW

The QuantiFluor® ssDNA System contains a fluorescent dye that enables sensitive quantitation of small amounts of single-stranded (ssDNA) in solution. Detecting and quantitating ssDNA is useful for a variety of molecular biology research applications. These include studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analysis of first-strand cDNAs, and quantitating bisulfate-treated DNA to study DNA methylation.

SENSITIVITY

For low-concentration samples, sensitivity of the QuantiFluor® ssDNA System is significantly increased (several thousand-fold) compared to absorbance at 260nm (NanoDrop® method).

LESS TEMPLATE ssDNA REQUIRED

The QuantiFluor® ssDNA System requires less DNA (200pg) than other methods (~1 µg). You save more of your precious sample for downstream assays.

EASE OF USE

System includes all the necessary reagents to quickly set up and quantitate ssDNA.

Offers an integrated solution with instrument pairing. Sets up easily on microplate or single-tube fluorometers from Promega.

INSTRUMENT COMPATIBILITY

Cat #E6105 Quantus™ Fluorometer
Using Blue Channel (Ex 460 nm, Em 515 - 575 nm)

Cat #E6070 or #E6080 GloMax®-Multi Jr
With Cat #E6071 Fluorescence Optical Kit - BLUE (Ex 460 nm, Em 515 - 570 nm)

Cat #E8032 or #E9032 GloMax®-Multi+
With Cat #E8051 Fluorescence Module and Fluorescence Optical Kit - BLUE (Ex 490, Em 510 - 570 nm)

Cat #E6090 QuantiFluor®-ST UV/Blue Channel (discontinued)
Using Blue Channel (Ex 460 nm, Em 515 - 575 nm)

Cat #E6105 QuantiFluor®-P (discontinued)
Using Blue Channel (Ex 460 nm, Em 515 - 575 nm) 

Quantus™ Fluorometer

11794MA_550_270

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