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Promega Corporation

GloMax®-Multi Jr Single Tube Multimode Reader

GloMax Multi Jr Single-Tube Multimode Reader

LUMINESCENCE APPLICATIONS

  • Luciferase Assays
  • ATP Assays
  • Chemiluminescent Immunoassays
  • Kinetics Assays
mod_st_lum_mod

The GloMax®-Multi Jr with a Luminescence Module is designed to deliver performance equivalent to dedicated single-tube luminometers while also offering the flexibility of a multi mode reader. To achieve sensitivity on par with that of a dedicated luminometer, the luminescence channel is separated from other measurement technologies and positioned directly below the sample well. These conditions maximize light capture for the best possible sensitivity. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised. The GloMax®-Multi Jr also has greater than 5 logs of dynamic range. This dynamic range is more than adequate to cover common luminescence applications, thus reducing the need to dilute samples.

FLUORESCENCE APPLICATIONS

  • DNA/RNA Quantitation
  • Gene Expression Assays
  • Enzyme Assays
  • Protein Quantitation

The GloMax®-Multi Jr with a Fluorescence Module is designed to deliver both high performance and flexibility. To achieve high performance, each Fluorescence Module utilizes powerful light-emitting diodes (LEDs) as excitation sources. LEDs have very specific light-output profiles which closely match the excitation profile of commonly used fluorescent molecules. LED usage increases sensitivity by fully exciting the fluorophore and reducing non-specific light leakage, a common problem when using broad-spectrum light sources. Additionally, the highly-sensitive GloMax®-Multi Jr uses a 90-degree fluorescent design to minimize light scatter and lower background resulting in high sensitivity.

GloMax Multi Jr Fluorescence Module

The GloMax®-Multi Jr as a fluorometer utilizes four easily exchangeable modules to measure the most popular fluorophores. In addition, custom modules can be made for non-standard applications.

To inquire about availability of custom optical kits click here to contact us. Please, note we cannot guarantee assay performance when using custom optical kits.

Fluorescence Module Typical Fluorophores
UV
(Ex 365 nm, Em 410 - 450 nm)
Hoechst Dye, 4-methyl-umbelliferone (4-MU)
Blue
(Ex 460 nm, Em 515 - 570 nm)
EGFP, rAcGFP, DNA Quantitation, RNA Quantitation, Fluorescein, Protein Quantitation
Green
(Ex 525 nm, Em 580 - 640 nm)
Rhodamine, Cy3, RFP
Red
(Ex 625 nm, Em 660 - 725 nm)
Cy5, Quant-iT™ RNA

ABSORBANCE APPLICATIONS

  • Protein Quantitation
  • Cell Turbidity, OD 600
GloMax Multi Jr Absorbance Module

The GloMax®-Multi Jr with the Absorbance Module provides measurements that are highly sensitive and cover a wide dynamic range. Because the optics are not shared with any other detection technology, the focused absorbance measurements yield outstanding performance. The absorbance channel has a large reading range of 0 - 4 OD with an accuracy that deviates less than 0.7%. The GloMax®-Multi Jr has three factory available filter paddles with filters for measuring 560, 600, and 750 nm. These filters accommodate the most common protein assays. Filter paddles can be easily exchanged in seconds. In addition, custom filter paddles can be readily made for non-standard applications.

To inquire about availability of custom optical kits click here to contact us. Please, note we cannot guarantee assay performance when using custom optical kits.

SPECIFICATIONS

GLOMAX®-Multi Jr
Available Detection Modes: Luminescence, Fluorescence, Absorbance
Read Type: Glow, Kinetic, Repeat
Sample Format: 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional), 0.5 ml PCR tube (optional, fluorescence), 1.5 ml microcentrifuge tube (Luminescence)
User Interface: Touch screen navigation and operation
Data Output: Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available.
External PC Requirements (optional): Compatible with 32- and 64-bit PCs using Windows® 7 Professional Service Pack 1 with either Microsoft® Office 2010 Home and Business Edition (32- and 64-bit versions) or Microsoft® Office 2007 (32-bit version)
Computer Interface: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate
Power: Instrument: 12V DC, 0.7A Max, AC Adapter: 100-240V AC, 50/60Hz, 0.3A
Auto Shutoff: Touch screen hibernates after 15 minutes of inactivity
Dimensions: 12.92” D x 10.44” W x 8.42” H (32.82 cm D x 26.52 cm W x 21.39 cm H)
Weight: ~6.5 lbs (2.95 kg)
Operating Temperature: 59 - 86 °F (15 - 30 °C)
Warranty: One year
Approvals: CE
Luminescence Module Specifications (Factory Installed)
Detector: Photomultiplier tube (PMT)
Spectral Range: 350 - 650 nm
Peak Wavelength: 420 nm
Detection Limit: 1 x 10-16 moles ATP or 1 x 10-20 moles Luciferase
Linear Dynamic Range: 5 logs
Sample Format: 1.5 ml microcentrifuge tubes
Fluorescence Module Specifications (User Installable)
Light Source: Wavelength-matched LED
Detector: Photodiode
Wavelength Selection: Snap-in Fluorescence Modules: UV (Ex 365 nm Em 410 - 450 nm), Blue (Ex 460 nm Em 515 - 570 nm), Green (Ex 525 nm, Em 580 - 640 nm), Red (Ex 625 nm, Em 660 - 725nm), UV-GFP (Ex 365 nm, Em 515 - 570nm)
Detection Limit: 6 ppt fluorescein, 450 pg/ml DNA Quantitation Dye
Linear Dynamic Range: 6 logs, assay dependent
Sample Format: 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional), 0.5 ml PCR tube (optional, 100 μl minimum)
Read Out: Fluorescence Standard Units, Direct Concentration
Calibration: 1 - 5 point calibration
Discrete Sample Average: Sample readings are averaged over 5 seconds to improve accuracy
Absorbance Module Specifications (User Installable)
Light Source: LED
Detector: Photodiode
Spectral Range: 450 - 750 nm
Wavelengths for Installed Filters: 560, 600, 750 nm
Photometric Measuring Range: 0 - 4.0 OD
Linear Dynamic Range: 0 - 2.5 OD, assay dependent
OD Accuracy: ±0.7%
OD Precision: ±0.5% at 1.0 OD

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