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Promega Corporation

GloMax®-Multi Jr Single Tube Multimode Reader

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A modular single tube multimode reader for luminescence, fluorescence, and absorbance Life Science applications.

GloMax Multi Jr Single-Tube Multimode Reader

The GloMax®-Multi Jr replaces the Turner BioSystems Modulus® Single Tube Multimode Reader. Same instrument... new look!

If you need higher throughput in your research, see our GloMax®-Multi+ Multimode Microplate Reader.

OVERVIEW

The GloMax®-Multi Jr Single Tube Multimode Reader is designed to provide the utmost flexibility. In addition to high performance, the GloMax®-Multi Jr blends user-friendly operation and a small footprint with flexible purchasing options. The result of this design is an instrument with superior performance that is easy to use, affordable, and can be customized to your laboratory's needs.

PERFORMANCE

The GloMax®-Multi Jr combines the superior performance expected from single-mode instruments with the functionality of multiple modes. To achieve high performance, the GloMax®-Multi Jr is designed with optical channels dedicated to each individual technology. Unlike other multimode systems, readings taken with the GloMax®-Multi Jr are not degraded by indirect fiber-optic transmission or crowded optical channels. Dedicated optical channeling ensures that the GloMax®-Multi Jr provides sensitivity and dynamic range on par with single-mode instruments.

EASE OF USE

The GloMax®-Multi Jr is designed to be put into use straight from the box without the need for special training. To achieve this plug-and-play usability, the GloMax®-Multi Jr combines a color touch screen with an intuitive user interface. The interface makes running samples and viewing data fast and simple while also maintaining the flexibility needed for advanced or custom protocols.

AFFORDABLE MODULARITY

The GloMax®-Multi Jr is a modular instrument that fits easily into most budgets. Purchase the technology or modes that you need now and add onto the system later as your needs expand. For example, the GloMax®-Multi Jr can be purchased as a Luminometer, then Fluorescence and/or Absorbance Modules can be purchased and added later. There's no service call or downtime. With the modular design, changing technologies can be as easy as snapping in a module and restarting the instrument.

SAMPLE FORMATS

For luminescence measurements, the GloMax®- Multi Jr accepts 1.5-ml microcentrifuge tubes. For fluorescence and absorbance measurements, the GloMax®-Multi Jr accepts standard 10 x 10 mm cuvettes.

PCR TUBE ADAPTER

An optional PCR Tube Adapter allows measurement of small fluorescence sample volumes (100 ul minimum) in 0.5 mL PCR tubes without sacrificing instrument sensitivity.

PCR Tube Adapter

MINICELL ADAPTER

For fluorescence measurements of sample volumes between 100 μl and 200 μl, a minicell adapter is available as an optional accessory.

Minicell Adapter

LID START

Adjust settings to start measurement immediately after the lid is closed.

TOUCHSCREEN

The touch screen allows simple navigation to set parameters, set calibrations, and start runs.

TROUBLESHOOTING

Easy access to HELP with the touch of a button.

DATA HANDLING

The GloMax®-Multi Jr stores up to 18 calibrations. Data from the last 20 measurements are displayed on the color LCD screen. Optional accessories for easy data exportation include spreadsheet Interface software or a thermal printer.

INTUITIVE USER INTERFACE

Luminescence
Choose from pre-loaded Promega protocols, saved protocols, or create your own custom protocol. Use the Instrument Control screen to edit parameters, then press MEASURE LUMINESCENCE.

Fluorescence
The GloMax®-Multi Jr can operate in raw fluorescence mode, or touch calibrate to create a new calibration or run a previously saved calibration.

Absorbance
For absorbance measurements, insert a blank control and press BLANK. Based on what you've selected on the touch screen, absorbance data is presented in either percent of transmission or absorbance units.

LUMINESCENCE APPLICATIONS

  • Luciferase Assays
  • ATP Assays
  • Chemiluminescent Immunoassays
  • Kinetics Assays
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The GloMax®-Multi Jr with a Luminescence Module is designed to deliver performance equivalent to dedicated single-tube luminometers while also offering the flexibility of a multi mode reader. To achieve sensitivity on par with that of a dedicated luminometer, the luminescence channel is separated from other measurement technologies and positioned directly below the sample well. These conditions maximize light capture for the best possible sensitivity. In addition, a low-noise photomultiplier tube ensures that collected light is not compromised. The GloMax®-Multi Jr also has greater than 5 logs of dynamic range. This dynamic range is more than adequate to cover common luminescence applications, thus reducing the need to dilute samples.

FLUORESCENCE APPLICATIONS

  • DNA/RNA Quantitation
  • Gene Expression Assays
  • Enzyme Assays
  • Protein Quantitation

The GloMax®-Multi Jr with a Fluorescence Module is designed to deliver both high performance and flexibility. To achieve high performance, each Fluorescence Module utilizes powerful light-emitting diodes (LEDs) as excitation sources. LEDs have very specific light-output profiles which closely match the excitation profile of commonly used fluorescent molecules. LED usage increases sensitivity by fully exciting the fluorophore and reducing non-specific light leakage, a common problem when using broad-spectrum light sources. Additionally, the highly-sensitive GloMax®-Multi Jr uses a 90-degree fluorescent design to minimize light scatter and lower background resulting in high sensitivity.

GloMax Multi Jr Fluorescence Module

The GloMax®-Multi Jr as a fluorometer utilizes four easily exchangeable modules to measure the most popular fluorophores. In addition, custom modules can be made for non-standard applications.

To inquire about availability of custom optical kits click here to contact us. Please, note we cannot guarantee assay performance when using custom optical kits.

Fluorescence Module Typical Fluorophores
UV
(Ex 365 nm, Em 410 - 450 nm)
Hoechst Dye, 4-methyl-umbelliferone (4-MU)
Blue
(Ex 460 nm, Em 515 - 570 nm)
EGFP, rAcGFP, DNA Quantitation, RNA Quantitation, Fluorescein, Protein Quantitation
Green
(Ex 525 nm, Em 580 - 640 nm)
Rhodamine, Cy3, RFP
Red
(Ex 625 nm, Em 660 - 725 nm)
Cy5, Quant-iT™ RNA

ABSORBANCE APPLICATIONS

  • Protein Quantitation
  • Cell Turbidity, OD 600
GloMax Multi Jr Absorbance Module

The GloMax®-Multi Jr with the Absorbance Module provides measurements that are highly sensitive and cover a wide dynamic range. Because the optics are not shared with any other detection technology, the focused absorbance measurements yield outstanding performance. The absorbance channel has a large reading range of 0 - 4 OD with an accuracy that deviates less than 0.7%. The GloMax®-Multi Jr has three factory available filter paddles with filters for measuring 560, 600, and 750 nm. These filters accommodate the most common protein assays. Filter paddles can be easily exchanged in seconds. In addition, custom filter paddles can be readily made for non-standard applications.

To inquire about availability of custom optical kits click here to contact us. Please, note we cannot guarantee assay performance when using custom optical kits.

SPECIFICATIONS

GLOMAX®-Multi Jr
Available Detection Modes: Luminescence, Fluorescence, Absorbance
Read Type: Glow, Kinetic, Repeat
Sample Format: 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional), 0.5 ml PCR tube (optional, fluorescence), 1.5 ml microcentrifuge tube (Luminescence)
User Interface: Touch screen navigation and operation
Data Output: Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available.
External PC Requirements (optional): Compatible with 32- and 64-bit PCs using Windows® 7 Professional Service Pack 1 with either Microsoft® Office 2010 Home and Business Edition (32- and 64-bit versions) or Microsoft® Office 2007 (32-bit version)
Computer Interface: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud rate
Power: Instrument: 12V DC, 0.7A Max, AC Adapter: 100-240V AC, 50/60Hz, 0.3A
Auto Shutoff: Touch screen hibernates after 15 minutes of inactivity
Dimensions: 12.92” D x 10.44” W x 8.42” H (32.82 cm D x 26.52 cm W x 21.39 cm H)
Weight: ~6.5 lbs (2.95 kg)
Operating Temperature: 59 - 86 °F (15 - 30 °C)
Warranty: One year
Approvals: CE
Luminescence Module Specifications (Factory Installed)
Detector: Photomultiplier tube (PMT)
Spectral Range: 350 - 650 nm
Peak Wavelength: 420 nm
Detection Limit: 1 x 10-16 moles ATP or 1 x 10-20 moles Luciferase
Linear Dynamic Range: 5 logs
Sample Format: 1.5 ml microcentrifuge tubes
Fluorescence Module Specifications (User Installable)
Light Source: Wavelength-matched LED
Detector: Photodiode
Wavelength Selection: Snap-in Fluorescence Modules: UV (Ex 365 nm Em 410 - 450 nm), Blue (Ex 460 nm Em 515 - 570 nm), Green (Ex 525 nm, Em 580 - 640 nm), Red (Ex 625 nm, Em 660 - 725nm), UV-GFP (Ex 365 nm, Em 515 - 570nm)
Detection Limit: 6 ppt fluorescein, 450 pg/ml DNA Quantitation Dye
Linear Dynamic Range: 6 logs, assay dependent
Sample Format: 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional), 0.5 ml PCR tube (optional, 100 μl minimum)
Read Out: Fluorescence Standard Units, Direct Concentration
Calibration: 1 - 5 point calibration
Discrete Sample Average: Sample readings are averaged over 5 seconds to improve accuracy
Absorbance Module Specifications (User Installable)
Light Source: LED
Detector: Photodiode
Spectral Range: 450 - 750 nm
Wavelengths for Installed Filters: 560, 600, 750 nm
Photometric Measuring Range: 0 - 4.0 OD
Linear Dynamic Range: 0 - 2.5 OD, assay dependent
OD Accuracy: ±0.7%
OD Precision: ±0.5% at 1.0 OD

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Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

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