Frequently Asked Questions
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What are the primary applications of the QuantiFluor®-ST Fluorometer?
The primary application for the QuantiFluor®-ST Fluorometer are nucleic acid and protein quantitation. A list of the applications include:
DNA Quantitation Dyes, Hoechst 33258
RNA Quantitation Dyes
Protein Quantitation Dyes
A dsDNA Dye System is available (Cat.# E2670) and includes all the necessary reagents to quickly set up and quantitate dsDNA using the UV/Blue channel on the QuantiFluor®-ST. This system is highly specific to dsDNA as it relates to ssDNA, RNA, protein and interfering compounds and is equal in sensitivity when compared to PicoGreen®.
What is the sensitivity of the QuantiFluor®-ST Fluorometer?
The sensitivity of the QuantiFluor®-ST is defined in terms of the minimum detection limits of dsDNA and RNA. The QuantiFluor®-ST Fluorometer can detect:
||Minimum Detection Limit
||Using a 10 × 10mm Cuvette
||Using a Minicell
|DNA Quantitation Dye
|DNA - Hoechst 33258 Dye
|RNA Quantitation Dye
|Protein Quantitation Dye
What is the optical configuration of the QuantiFluor®-ST Mini-Fluorometer?
The QuantiFluor®-ST has two dedicated optical channels, UV and BLUE channels.
UV: Ex 365nm, Em 440–470nm
BLUE: Ex 460nm, Em 515–575nm
What size sample tube/cuvette is standard with the QuantiFluor®-ST Fluorometer?
The standard cuvette size for the QuantiFluor®-ST Fluorometer is 10 × 10mm plastic cuvettes. With the optional Minicell Adapter, 50–250µl minicell vial can be used. An optional PCR Tube Adapter allows measurement of small fluorescence sample volumes (100µl minimum) in 0.5ml PCR tubes without sacrificing instrument sensitivity.
What is the Minicell Adapter?
The QuantiFluor®-ST Minicell Adapter is designed to accommodate the measurement of small volume samples. The design of the focusing lenses allows you to measure fluorescence samples in a much reduced assay volume without losing sensitivity.
Do the sample tubes/cuvettes require minimum volumes? If so, what are they?
Sample tubes do require minimum volumes so that enough sample is exposed to the incoming light. The minimum volumes are as follows:
|10 × 10mm
What kind of cuvettes should I use with the QuantiFluor®-ST Fluorometer?
Methacrylate or polystyrene 10 × 10mm cuvette or 10 × 10mm adapter that is designed for fluorescence measurement is appropriate. Due to the natural fluorescence properties of polystyrene, this material is not recommended for UV applications. The following sample tubes and cuvettes are available for purchase from Promega:
10 × 10mm Square Polystyrene Cuvette (3.5ml)
10 × 10mm Square Methacrylate Cuvette (3.5ml)
Minicell Borosilicate Glass (50–250ml)
Can I use a quartz or glass cuvette?
The sample compartment cannot accept glass or quartz cuvettes.
Does the QuantiFluor®-ST Fluorometer have changeable optical filters?
No, the QuantiFluor®-ST Fluorometer does not have changeable optical filters. If the optical configuration of the QuantiFluor®-ST Fluorometer does not fit your application requirement, please see information about the GloMax®-Multi Jr Single Tube Multimode Reader.
What is the dynamic range of the QuantiFluor®-ST Fluorometer?
The dynamic range of the QuantiFluor®-ST Fluorometer is 4 logs, assay-dependent.
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