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Promega Corporation

DNA and RNA Purification / Extraction Selector

  • DNA Purification
  • RNA Purification
Sample Type Sample Volume
(or amount)
Time to Process
(minutes)
Format (Auto/Manual) Intended Use Then Use:
 
Blood
≤200µl whole blood 30 or less ≤30 M Research ReliaPrep™ Blood gDNA Miniprep System
<350µl whole blood 96 samples in <150 <150 A Research ReliaPrep™ 96 gDNA Miniprep HT System
50µl to 10ml whole blood About 60 ~60 M Research Wizard® Genomic DNA Purification Kit
50–400µl whole blood Up to 16 samples <40 A Research Maxwell® 16 Blood DNA Purification Kit
Up to 300µl whole blood Up to 16 samples ~60 A Research Maxwell® 16 LEV Blood DNA Purification Kit
Up to 250µl Buffy Coat Up to 16 samples 50 A Research Maxwell® 16 Blood DNA Purification Kit
1–10ml whole blood 32 samples <240 A Research ReliaPrep™ HT gDNA Isolation System
Up to 300µl whole blood Up to 16 samples 40 A Research Maxwell® RSC Blood DNA Kit
50–300µl human whole blood 1–16 samples 40 A IVD Maxwell® CSC Blood DNA Kit
 
Tissue
Up to 20mg or 1.2cm (mouse tail)
or one buccal swab
20–30* 20–30 M Research ReliaPrep™ gDNA Tissue Miniprep System
11mg (mouse liver)
0.5–1cm (mouse tail)
40mg (tomato leaf)
About 60* ~60 M Research Wizard® Genomic DNA Purification Kit
Up to 20mg or 1.2cm (mouse tail) 20–30* 20–30 M Research Wizard® SV Genomic DNA Purification System
Up to 50mg (various) Up to 18 samples 45 A Research Maxwell® 16 Tissue DNA Purification Kit
Up to 50mg (mouse tail) Up to 16 samples 45 A Research Maxwell® 16 Mouse Tail DNA Purification Kit
 
FFPE
FFPE tissue samples ranging in size from 0.1mm3 to 2.0mm3. Up to 16 samples ~60 A Research Maxwell® RSC DNA FFPE Purification kit
5–50µm tissue sections 150 including
deparaffinization
~150 M Research ReliaPrep™ FFPE gDNA Miniprep System
1–10 5µm tissue sections Up to 16 samples
in 30**
30 A Research Maxwell® FFPE Plus LEV DNA Purification Kit
human breast, lung or colon tissue samples (0.1mm3 to 2.0mm3 tissue sections) 1–16 samples <50 A IVD Maxwell® CSC DNA FFPE Kit
 
Cells
350 or 700µl (Oragene sample) 96 samples in <150 <150 A Research ReliaPrep™ 96 gDNA Miniprep HT System
1×104 to 8×106 culture cells About 60 ~60 M Research Wizard® Genomic DNA Purification Kit
1×104 to 5×106
culture cells
20–30* 20–30 M Research Wizard® SV Genomic DNA Purification System
10 to 1×104 cells Up to 16 samples
in 30–45
30–45 A Research Maxwell® 16 Cell DNA Purification Kit
10 to 1×104 cells Up to 16 samples
in 30–45
30–45 A Research Maxwell® 16 Cell LEV DNA Purification Kit
1 to 2 buccal swabs Up to 16 samples
in 40
40 A Research Maxwell® 16 Buccal Swab LEV DNA Purification Kit
104 to 106 cells Up to 16 samples
in <25
25 A Research Maxwell® RSC Cell DNA Purification Kit
 
Plant
Up to 20mg leaf tissue Up to 16 samples
in ~40
~40 A Research Maxwell® 16 LEV Plant DNA Kit
 
Viral
100–300µl plasma or serum Up to 16 samples
in < 45
<45 A Research Maxwell® 16 Viral Total Nucleic Acid Purification Kit
100–300µl plasma or serum Up to 16 samples
in < 40
<40 A Research Maxwell® RSC Viral Total Nucleic Acid Purification Kit
 
Plasmids
600µl to 3ml bacterial culture About 10 ~10 M Research PureYield™ Plasmid Miniprep System
25 to 100ml About 40 ~40 M Research PureYield™ Plasmid Midiprep System
Up to 250ml About 60 ~60 M Research PureYield™ Plasmid Maxiprep System
1–10ml About 45 ~45 M Research Wizard® Plus SV Minipreps DNA Purification System
4.0 OD600 culture 60 or less per plate ≤60 M/A Research Wizard® SV 96 Plasmid DNA Purification System
6.0 OD600 culture Less than 90 per plate <90 A Research Wizard® MagneSil® Plasmid DNA Purification System
6.0 OD600 culture 45 or less per plate ≤45 A Research Wizard® MagneSil® Tfx™ System
 
Fragments
Up to 350mg agarose gel slice or
up to 1ml enzymatic reaction
about 15 ~15 M Research Wizard® SV Gel and PCR Clean-Up System
Up to 200µl PCR reaction
20 20 M Research Wizard® SV 96 PCR Clean-Up System
40–75 40–75 A Research
20µl Sequencing reaction 35–90 35–90 A Research Wizard® MagneSil® Sequencing Reaction Clean-Up System
* Processing time after Lysate creation
** Processing time after either overnight or one hour ProK digestion
† Research: Products listed for this application are intended for research use only and not for use in diagnostic applications.
   IVD: Products listed for this application are intended for in vitro diagnostic use. This product is only available in certain countries.
Sample Type Sample Volume
(or amount)
Time to Process
(minutes)
Format (Auto/Manual) Intended Use Then Use:
 
Blood
100µl blood About 60 ~60 M Research SV RNA Isolation System
Leukocytes from 1ml blood About 60 ~60 M Research SV RNA Isolation System
10–20ml blood Less than 120 <120 M Research PureYield™ RNA Midiprep System
2ml stabilized blood Up to 16 samples ~70 A Research Maxwell® 16 Total RNA Purification Kit
Up to 20µl blood About 30 ~30 A Research MagneSil® Total RNA mini-Isolation System
2.5ml blood About 60 ~60 A Research Maxwell® 16 simplyRNA Blood Kit
2.5ml blood Up to 60 samples <60 A Research Maxwell® RSC simplyRNA Blood Kit
 
Tissue
Up to 20mg About 30 ~30 M Research ReliaPrep™ RNA Tissue Miniprep System
15–60mg About 60 60 M Research SV RNA Isolation System
150–300mg Less than 120 <120 M Research PureYield™ RNA Midiprep System
>3mg Less than 60 <60 M/A Research SV 96 RNA Isolation System
Up to 20mg of tissue Up to 16 samples
in <60
<60 A Research Maxwell® 16 LEV simplyRNA Tissue Kit
Up to 20mg of tissue Up to 16 samples <60 A Research Maxwell® RSC simplyRNA Tissue Kit
≤2mg tissue lysate in 100µl About 30 ~30 A Research MagneSil® Total RNA mini-Isolation System
 
FFPE
5-10µm sections with a size
range of 20mm2 to 200mm2 for a total of up to 2.0mm3.
Up to 16 samples ~60 A Research Maxwell® RSC RNA FFPE Purification kit
5µm tissue sections About 60 minutes** ~60 A Research Maxwell® 16 LEV RNA FFPE Kit
5–50µm tissue selections About 90 including deparaffinization ~90 M Research ReliaPrep™ FFPE Total RNA Miniprep System
human breast, lung or colon tissue samples (0.1mm3 to 2.0mm3 tissue sections) 1–16 samples <50 A IVD Maxwell® CSC RNA FFPE Kit
 
Cells
100 to 5 × 106 tissue culture cells About 30 ~30 M Research ReliaPrep™ RNA Cell Miniprep System
≤5 × 106 tissue culture cells About 60 ~60 M Research SV RNA Isolation System
1-5 × 107 cells Less than 120 <120 M Research PureYield™ RNA Midiprep System
≤5 × 106 tissue culture cells Less than 60 <60 M/A Research SV 96 RNA Isolation System
≤1 × 105 tissue culture cells About 30 ~30 A Research MagneSil® Total RNA mini-Isolation System
Up to 5 × 106 cells Up to 16 samples <60 A Research Maxwell® RSC simplyRNA Cells Purification Kit
Up to 2 × 106 cells Up to 16 samples
in <60
<60 A Research Maxwell® 16 LEV simplyRNA Cells Kit
 
Plant
Up to 100mg leaf tissue Up to 16 samples <60 A Research Maxwell® 16 Plant RNA Kit
Leaf tissue up to 300mg Less than 120 <120 M Research PureYield™ RNA Midiprep System
 
Viral
100–300µl plasma or serum Up to 16 samples
in < 45
<45 A Research Maxwell® 16 Viral Total Nucleic Acid Purification Kit
100–300µl plasma or serum Up to 16 samples
in < 40
<40 A Research Maxwell® RSC Viral Total Nucleic Acid Purification Kit
** Processing time after either overnight or one hour ProK digestion
† Research: Products listed for this application are intended for research use only and not for use in diagnostic applications.
   IVD: Products listed for this application are intended for in vitro diagnostic use. This product is only available in certain countries..

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