Promega Corporation

DNA and RNA Purification / Extraction Selector

  • DNA Purification
  • RNA Purification
Sample Type Sample Volume
(or amount)		 Time to Process
(minutes)		 Format (Auto/Manual) Then Use:
 
Blood
≤200µl 30 minutes or less ≤30 M ReliaPrep™ Blood gDNA Miniprep System
50µl to 10ml About 60 minutes 60 M Wizard® Genomic DNA Purification Kit
50-400µl Up to 16 samples
in 30-45 minutes		 30-45 A Maxwell® 16 Blood DNA Purification Kit
Up to 300µl whole blood Up to 16 samples
in 40 minutes		 40 A Maxwell® 16 LEV Blood DNA Purification Kit
Up to 500µl Buffy Coat Up to 16 samples
in 30-45 minutes		 30-45 A Maxwell® 16 Blood DNA Purification Kit
3-10ml 96 samples in
less than 8 hours		 ≤8 hrs A ReliaPrep™ HT gDNA Isolation System
 
Tissue
1.2cm (mouse tail) or Up to 20mg
One buccal swab		 20-30 minutes* 20-30 M ReliaPrep™ gDNA Tissue Miniprep System
11mg (mouse liver)
0.5-1cm (mouse tail)
40mg (tomato leaf)		 About 60 minutes* 60 M Wizard® Genomic DNA Purification Kit
1.2cm (mouse tail) or Up to 20mg 20-30 minutes* 20-30 M Wizard® SV Genomic DNA Purification System
Up to 50mg (various) Up to 18 samples
in 30-45 minutes		 30-45 A Maxwell® 16 Tissue DNA Purification Kit
Up to 50mg (mouse tail) Up to 16 samples
in <45 minutes		 <45 A Maxwell® 16 Mouse Tail DNA Purification Kit
 
FFPE
5-50µm tissue sections About 150 minutes
including deparaffinization		 ~2.5 hrs M ReliaPrep™ FFPE gDNA Miniprep System
1-10 5µm tissue sections Up to 16 samples
in 30 minutes***		 30 A Maxwell® FFPE Plus LEV DNA Purification Kit
 
Cells
1×104 to 8×106 culture cells About 60 minutes 60 M Wizard® Genomic DNA Purification Kit
1×104 to 5×106
culture cells		 20-30 minutes* 20-30 M Wizard® SV Genomic DNA Purification System
10 to 1×104 cells Up to 16 samples
in 30-45 minutes		 30-45 A Maxwell® 16 Cell DNA Purification Kit
10 to 1×104 cells Up to 16 samples
in 30-45 minutes		 30-45 A Maxwell® 16 Cell LEV DNA Purification Kit
1 to 2 buccal swabs Up to 16 samples
in 40 minutes		 40 A Maxwell® 16 Buccal Swab LEV DNA Purification Kit
 
Plasmids
600µl to 3ml bacterial culture About 10 minutes 10 M PureYield™ Plasmid Miniprep System
25 to 100ml About 40 minutes 40 M PureYield™ Plasmid Midiprep System
Up to 250ml About 60 minutes 60 M PureYield™ Plasmid Maxiprep System
1-10ml About 45 minutes 45 M Wizard® Plus SV Minipreps DNA Purification System
4OD600 culture 60 minutes or less/ plate ≤60 M/A Wizard® SV 96 Plasmid DNA Purification System
6OD600 culture Less than 90 minutes/ plate <90 A Wizard® MagneSil® Plasmid DNA Purification System
6OD600 culture About 45 minutes/ plate ≤45 A Wizard® MagneSil® Tfx™ System
 
Fragments
Up to 350mg agarose gel slice Or
Up to 1ml enzymatic reaction		 ~15 minutes 15 M Wizard® SV Gel and PCR Clean-Up System
Up to 200µl PCR reaction
20 minutes 20 M Wizard® SV 96 PCR Clean-Up System
40-75 minutes 40-75 A
20µl Sequencing reaction 35-90 minutes 35-90 A Wizard® MagneSil® Sequencing Reaction Clean-Up System
* Processing time after Lysate creation
** Processing time after overnight ProK digestion
*** Processing time after either overnight or one hour ProK digestion
Sample Type Sample Volume
(or amount)		 Time to Process
(minutes)		 Format (Auto/Manual) Then Use:
 
Blood
100µl blood About 60 minutes 60 M SV RNA Isolation System
Leukocytes from 1ml blood About 60 minutes 60 M SV RNA Isolation System
10-20ml blood Less than 2 hours <2 hrs M PureYield™ RNA MidiPrep System
2ml stabilized blood Up to 16 samples
in 30 minutes		 30 A Maxwell® 16 Total RNA Purification Kit
Up to 20µl blood About 30 minutes 30 A MagneSil® Total RNA mini-Isolation System
 
Tissue
15-60mg About 60 minutes 60 M SV RNA Isolation System
150-300mg Less than 2 hours <2 hrs M PureYield™ RNA MidiPrep System
Less than 1 hour <1 hr M/A SV 96 RNA Isolation System
Up to 20mg tissue Up to 16 samples
in <1 hour		 <1 hr A Maxwell® 16 LEV simplyRNA Tissue Kit
≤2mg tissue lysate in 100µl About 30 minutes 30 A MagneSil® Total RNA mini-Isolation System
 
FFPE
5-50µm tissue selections About 90 minutes including deparaffinization 90 M ReliaPrep™ FFPE Total RNA Miniprep System
 
Cells
≤5 × 106 tissue culture cells About 60 minutes 60 M SV RNA Isolation System
1-5 × 107 cells Less than 2 hours <2 hrs M PureYield™ RNA MidiPrep System
≤5 × 106 tissue culture cells Less than 1 hour <1 hr M/A SV 96 RNA Isolation System
≤1 × 105 tissue culture cells About 30 minutes 30 A MagneSil® Total RNA mini-Isolation System
Up to 2×106 cells Up to 16 samples
in <1 hour		 <1 hr A Maxwell® 16 LEV simplyRNA Cells Kit
 
Plant
Leaf tissue up to 300mg Less than 2 hours <2 hrs M PureYield™ RNA MidiPrep System

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