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Promega Corporation

DNA and RNA Purification / Extraction Selector

  • DNA Purification
  • RNA Purification
Sample Type Sample Volume
(or amount)
Time to Process
(minutes)
Format (Auto/Manual) Then Use:
 
Blood
≤200µl 30 or less ≤30 M ReliaPrep™ Blood gDNA Miniprep System
<350µl 96 samples in <150 <150 A ReliaPrep™ 96 gDNA Miniprep HT System
50µl to 10ml About 60 ~60 M Wizard® Genomic DNA Purification Kit
50–400µl Up to 16 samples
in 30–45
30–45 A Maxwell® 16 Blood DNA Purification Kit
Up to 300µl whole blood Up to 16 samples
in 40
40 A Maxwell® 16 LEV Blood DNA Purification Kit
Up to 500µl Buffy Coat Up to 16 samples
in 30–45
30–45 A Maxwell® 16 Blood DNA Purification Kit
1–10ml 32 samples in
less than 240
<240 A ReliaPrep™ HT gDNA Isolation System
 
Tissue
1.2cm (mouse tail) or Up to 20mg
or One buccal swab
20–30* 20–30 M ReliaPrep™ gDNA Tissue Miniprep System
11mg (mouse liver)
0.5–1cm (mouse tail)
40mg (tomato leaf)
About 60* ~60 M Wizard® Genomic DNA Purification Kit
1.2cm (mouse tail) or Up to 20mg 20–30* 20–30 M Wizard® SV Genomic DNA Purification System
Up to 50mg (various) Up to 18 samples
in 30–45
30–45 A Maxwell® 16 Tissue DNA Purification Kit
Up to 50mg (mouse tail) Up to 16 samples
in <45
<45 A Maxwell® 16 Mouse Tail DNA Purification Kit
 
FFPE
5–50µm tissue sections 150 including
deparaffinization
~150 M ReliaPrep™ FFPE gDNA Miniprep System
1–10 5µm tissue sections Up to 16 samples
in 30**
30 A Maxwell® FFPE Plus LEV DNA Purification Kit
 
Cells
350 or 700µl (Oragene sample) 96 samples in <150 <150 A ReliaPrep™ 96 gDNA Miniprep HT System
1×104 to 8×106 culture cells About 60 ~60 M Wizard® Genomic DNA Purification Kit
1×104 to 5×106
culture cells
20–30* 20–30 M Wizard® SV Genomic DNA Purification System
10 to 1×104 cells Up to 16 samples
in 30–45
30–45 A Maxwell® 16 Cell DNA Purification Kit
10 to 1×104 cells Up to 16 samples
in 30–45
30–45 A Maxwell® 16 Cell LEV DNA Purification Kit
1 to 2 buccal swabs Up to 16 samples
in 40
40 A Maxwell® 16 Buccal Swab LEV DNA Purification Kit
 
Plasmids
600µl to 3ml bacterial culture About 10 ~10 M PureYield™ Plasmid Miniprep System
25 to 100ml About 40 ~40 M PureYield™ Plasmid Midiprep System
Up to 250ml About 60 ~60 M PureYield™ Plasmid Maxiprep System
1–10ml About 45 ~45 M Wizard® Plus SV Minipreps DNA Purification System
4 x OD600 culture 60 or less per plate ≤60 M/A Wizard® SV 96 Plasmid DNA Purification System
6 x OD600 culture Less than 90 per plate <90 A Wizard® MagneSil® Plasmid DNA Purification System
6 x OD600 culture 45 or less per plate ≤45 A Wizard® MagneSil® Tfx™ System
 
Fragments
Up to 350mg agarose gel slice or
up to 1ml enzymatic reaction
about 15 ~15 M Wizard® SV Gel and PCR Clean-Up System
Up to 200µl PCR assay
20 20 M Wizard® SV 96 PCR Clean-Up System
40–75 40–75 A
20µl Sequencing reaction 35–90 35–90 A Wizard® MagneSil® Sequencing Reaction Clean-Up System
* Processing time after Lysate creation
** Processing time after either overnight or one hour ProK digestion
Sample Type Sample Volume
(or amount)
Time to Process
(minutes)
Format (Auto/Manual) Then Use:
 
Blood
100µl blood About 60 ~60 M SV RNA Isolation System
Leukocytes from 1ml blood About 60 ~60 M SV RNA Isolation System
10–20ml blood Less than 120 <120 M PureYield™ RNA Midiprep System
2ml stabilized blood Up to 16 samples
in 30
30 A Maxwell® 16 Total RNA Purification Kit
Up to 20µl blood About 30 ~30 A MagneSil® Total RNA mini-Isolation System
2.5ml blood About 60 ~60 A Maxwell® 16 simplyRNA Blood Kit
 
Tissue
Up to 20mg About 30 ~30 M ReliaPrep™ RNA Tissue Miniprep System
15–60mg About 60 60 M SV RNA Isolation System
150–300mg Less than 120 <120 M PureYield™ RNA Midiprep System
>3mg Less than 60 <60 M/A SV 96 RNA Isolation System
Up to 20mg of tissue Up to 16 samples
in <60
<60 A Maxwell® 16 LEV simplyRNA Tissue Kit
≤2mg tissue lysate in 100µl About 30 ~30 A MagneSil® Total RNA mini-Isolation System
 
FFPE
5–50µm tissue selections About 90 including deparaffinization ~90 M ReliaPrep™ FFPE Total RNA Miniprep System
 
Cells
100 to 5 × 106 tissue culture cells About 30 ~30 M ReliaPrep™ RNA Cell Miniprep System
≤5 × 106 tissue culture cells About 60 ~60 M SV RNA Isolation System
1-5 × 107 cells Less than 120 <120 M PureYield™ RNA Midiprep System
≤5 × 106 tissue culture cells Less than 60 <60 M/A SV 96 RNA Isolation System
≤1 × 105 tissue culture cells About 30 ~30 A MagneSil® Total RNA mini-Isolation System
Up to 2 × 106 cells Up to 16 samples
in <60
<60 A Maxwell® 16 LEV simplyRNA Cells Kit
 
Plant
Leaf tissue up to 300mg Less than 120 <120 M PureYield™ RNA Midiprep System

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