Discontinued Vectors

EMBL3 Genomic Cloning

Lambda replacement vector derived from lambda 1059.

• For sequence see GenBank® Acc.#: U02453 (right arm) and U02425 (left arm)

EMBL4 Genomic Cloning

Lambda replacement vector derived from lambda 1059.

• Identical to EMBL3 except that multiple cloning region is in the reverse orientation. EMBL4 has same stuffer fragment as EMBL3.
• For sequence see GenBank® Acc.#: None

HaloTag® pHT2 Vector (discontinued, 9/09)

• For sequence see GenBank® Acc.#: AY773970

pFC8A HaloTag® CMV Flexi® Vector (discontinued, 5/08)

• For sequence see GenBank® Acc.#: DQ137254

pFC8K HaloTag® CMV Flexi® Vector (discontinued, 5/08)

• For sequence see GenBank® Acc.#: DQ137255

LambdaGem-11 Genomic Cloning

Multifunctional replacement type genomic cloning vector that accepts DNA fragments of 9-23kb.

• For sequence see GenBank® Acc.#: U02427 (right arm)and U02426 (left arm)

LambdaGem-12 Genomic Cloning

Lambda replacement vector derived from lambda 1059.

• LambdaGem-12 arms are identical to EMBL3 arms (above) except that they have different multiple cloning regions containing T7 and SP6 RNA polymerase promoters and recognition sites listed below. LambdaGem-12 has a different stuffer fragment than EMBL3.

Lambda gt10

cDNA cloning vector.

• For sequence see GenBank® Acc.#: U02447

Lambda gt11

cDNA cloning vector.

• For sequence see GenBank® Acc.#: None

Lambda gt11 Sfi-Not

cDNA cloning vector.

pALTER®-1

Contains an multiple cloning site flanked by opposing SP6 and T7 RNA polymerase promoters, and inserted into the lacZ α-peptide-encoding DNA.

• For sequence see GenBank® Acc.#: X65334

pALTER®-Ex1

Contains a novel polylinker that allows protein expression in vivo and in vitro.

• For sequence see GenBank® Acc.#: U47102

pALTER®-Ex2

Contains the same cloning sites and transcription/translation signals as pALTER®-EX1, but used the p15a origin or replication.

• For sequence see GenBank® Acc.#: U47103

pCAT®-Basic

• For sequence see GenBank® Acc.#: X65322

pCAT®-Control

• For sequence see GenBank® Acc.#: X65321

pCAT®-Enhancer

• For sequence see GenBank® Acc.#: X65319

pCAT®-Promoter

• For sequence see GenBank® Acc.#: X65320

pET-5a

Bacterial expression.

• Sequence (.txt file) 

pET-5b

Bacterial expression.

• Sequence (.txt file) 

pET-5c

Bacterial expression.

• Sequence (.txt file) 

pET-9a

Bacterial expression.

• Sequence (.txt file) 

pET-9b

Bacterial expression.

• Sequence (.txt file) 

pET-9c

Bacterial expression.

• Sequence (.txt file) 

pGEM®-1

• For sequence see GenBank® Acc.#: X65300

pGEM®-2

• For sequence see GenBank® Acc.#: X65301

pGEM®-3

• For sequence see GenBank® Acc.#: X65302

pGEM®-4 Vector

• For sequence see GenBank® Acc.#: X65303

pGEM®-5Zf(-)

Cloning, in vitro transcription.

• For sequence see GenBank® Acc.#: X65309

pGEM®-13Zf(+)

Cloning, in vitro transcription, ssDNA synthesis.

• For sequence see GenBank® Acc.#: X65315

pGEM®-15Zf(–)

Cloning.

• Sequence (.txt file) 

pGEMEX®-1

Designed for cloning, as templates for in vitro transcription and for production of ssDNA.

• For sequence see GenBank® Acc.#: X65317

pGEMEX®-2

Designed for cloning, as templates for in vitro transcription and for production of ssDNA.

• For sequence see GenBank® Acc.#: X65318

phRG-B (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362550

phRG-TK (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362551

phRL-CMV (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362549

phRL-null (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362546

phRL-SV40 (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362548

phRL-TK (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362545
• MCR restriction enzymes: None

phRL-TK(Int–) (discontinued, 1/08)

Mammalian expression and reporter vector.

• For sequence see GenBank® Acc.#: AF362547

PinPoint™ Control/PinPoint™ Xa Control

Bacterial expression.

• Sequence (.txt file)

PinPoint™ Xa-1 Vector

Bacterial expression.

• For sequence, see GenBank® Acc.#: U47626

PinPoint™ Xa-2

Bacterial expression.

• Vector map and sequence are nearly identical to PinPoint™ Xa-1 (above), but contain one additional adenosine at position 394.

PinPoint™ Xa-3

Bacterial expression.

• Vector map and sequence are nearly identical to PinPoint™ Xa-1 (above), but contain two additional adenosines at position 394.

PinPoint™ Xa-1 T-Vector

This vector is designed for cloning PCR products.

• Sequence (.txt file)

psiLentGene™ Basic

psiLentGene™ Hygromycin

psiLentGene™ Neomycin

psiLentGene™ Puromycin

pSP64

Cloning, in vitro transcription.

pSP65

Cloning, in vitro transcription.

pSP70

Cloning, in vitro transcription.

pSP-luc+

Luciferase cassette vector.

psiSTRIKE™ Basic

Designed for in vivo expression of siRNAs.

psiSTRIKE™ hMGFP

Designed for in vivo expression of short hairpin RNAs.

psiSTRIKE™ Hygromycin

Designed for in vivo expression of siRNAs.

psiSTRIKE™ Neomycin

Designed for in vivo expression of siRNAs.

psiSTRIKE™ Puromycin

Designed for in vivo expression of siRNAs.