We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation

Improve Promoter Analysis with Luciferase Reporters


The pGL4.10 [luc2] Vector (Cat. #E6651) contains a multiple cloning site for insertion of promoter sequences.


The pGL4.74 [hRluc/TK] Vector (Cat. #E6921) ready to use as a control vector in Dual-Luciferase® Reporter experiments.

  • Promoter analysis seeks to understand why a particular gene responds to a particular environmental condition or treatment.
  • Originally, promoter dissection involved deletion analysis of the proximal promoter region until the gene response to the condition or treatment changed or was eliminated.
  • Today, bioinformatics allows researchers to make "educated guesses" about what regions of the promoter will be important for a particular response.
  • Promoter analysis is being used to ask broad questions, like why do certain polymorphisms persist in the genome, what makes a primate different from a human when the genes are identical, etc.
  • The pGL4 vectors represent the latest technology for promoter analysis.
  • We dramatically reduced background by removing hundreds of potential transcription factor binding sites from the vector backbone.
  • The re-engineered luciferase gene offers unparalleled sensitivity because the signal produced from the luciferase reaction is 200-fold brighter signal than that from the native luciferase gene.

Long Terminal Repeat Promoter Analysis of HIV-1


Treating T-cells infected with HIV-1 involves activating the provirus that has integrated into the genome so that these cells can be "marked" for treatment. However, activating the provirus triggers the NF-κB pathway and activates the T-cell, allowing the virus to replicate and infect additional cells. The authors of this study screened for factors that could activate a HIV-1 promoter in which the NF-κB sites were mutated.

Yang, H-C. et al. (2009) PNAS 106, 6321–6.


Above downloads in PDF format

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.