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ApoTox-Glo™ Triplex Assay

Predictive Mechanism of Toxicity Determination

  • Measure viability, cytotoxicity, and apoptosis in the same sample well: Accurately determine mechanism of cell death in the same sample well, save time and use less cells.
  • Easily implement: The assay follows a simple sequential "add-mix-measure" protocol.
  • Normalize data with a built-in control: The ratio of the number of live cells/number of dead cells is independent of cell number and normalizes data, making it more comparable well-to-well, plate-to-plate, and day-to-day.
  • Flexible and easily automated: Component volumes can be scaled to meet throughput needs, and reagents are robust for automation in 96- to 1536-well plate formats.

Predictive Mechanism of Toxicity Determination

6601MB

Measure viability, cytotoxicity and apoptosis in the same sample well

8141MA_8140MA


Primary Necrosis

  • Ionomycin treatment of Jurkat cells for 6 hours.
  • Dose-dependent decrease in viability
  • No caspase-3/7 activation


Apoptosis

  • Staurosporine treatment of Jurkat cells for 6 hrs.
  • Dose-dependent decrease in viability
  • Increase in caspase-3/7 activation
7124ma

Easily implement and adapt to multiwell formats

Simple add-mix-measure protocol and scalable assay reagents make the assay easy to implement and adaptable for 96- to 1536-well formats.

8141mb

Normalize data

The ratio of number of live cells to number of dead cells is independent of cell number and normalizes the data. This normalization makes results more comparable well-to-well, plate-to-plate and day-to-day.

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