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Promega Corporation

Tth DNA PolymerasePCR Satisfaction Guaranteed

Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at ...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Tth DNA Polymerase

100u
5u/μl M2101 $ 95.00 Add to cart

Tth DNA Polymerase

500u
5u/μl M2105 $ 390.00 Add to cart


Source

Thermus thermophilus HB-8.

Storage Buffer

10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA, 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTPs into TCA-insoluble material in 30 minutes at 70°C.

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