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Promega Corporation

Tth DNA PolymerasePCR Satisfaction Guaranteed

Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at ...

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Tth DNA Polymerase

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Tth DNA Polymerase

 Components

  • MgCl2

    A351B1 x 750μl
  • Thermophilic DNA Polymerase 10X Buffer

    M190A1 x 750μl
  • Tth DNA Polymerase

    M210A1 x 100u
  • MnCl2

    M212A1 x 750μl
  • RT 10X Buffer

    M213A1 x 750μl
  • Chelate 10X Buffer

    M214A1 x 750μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100u
5u/μl M2101 $ 95.00 Add to cart

Tth DNA Polymerase

Close Window

Tth DNA Polymerase

 Components

  • MgCl2

    A351B1 x 750μl
  • Thermophilic DNA Polymerase 10X Buffer

    M190A1 x 750μl
  • Tth DNA Polymerase

    M210B1 x 500u
  • MnCl2

    M212A1 x 750μl
  • RT 10X Buffer

    M213A1 x 750μl
  • Chelate 10X Buffer

    M214A1 x 750μl
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500u
5u/μl M2105 $ 390.00 Add to cart


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Source

Thermus thermophilus HB-8.

Storage Buffer

10mM Tris-HCl (pH 7.5 at 25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA, 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTPs into TCA-insoluble material in 30 minutes at 70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M2101, M2105 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

M2101, M2105 NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US patents 6,127,155, 5,561,058, 5,693,517 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

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