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Promega Corporation

Reverse Transcription System

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 sepa...

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Reverse Transcription System

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Reverse Transcription System

 Components

  • MgCl2

    A351H1 x 1.2ml
  • Reverse Transcription 10X Buffer

    A356A1 x 1.4ml
  • Oligo(dT)15 Primer

    C110B1 x 50μg
  • dNTP Mix

    C114B1 x 320μl
  • Random Primers

    C118B1 x 50μg
  • 1.2kb Kanamycin Positive Control RNA

    C138A1 x 5μg
  • AMV Reverse Transcriptase (HC)

    M900B1 x 1,500u
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
  • Nuclease-Free Water

    P119E1 x 13ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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Already a Helix Customer?

Request to add this product to your Helix »


100 reactions
- A3500 $ 480.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

Magnesium Chloride Solution

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Magnesium Chloride Solution

  Components

  • MgCl2

    A351B2 x 750μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1.5ml
25mM A3511 $ 35.00 Add to cart

Reverse Transcription 10X Buffer

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Reverse Transcription 10X Buffer

  Components

  • Reverse Transcription 10X Buffer

    A356A1 x 1.4ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1.4ml
- A3561 $ 35.00 Add to cart

Storage Conditions

Store at –20°C. Store Positive Control RNA at –70°C.

For product intended use please see Patents & Disclaimers tab.

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Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.