GoScript™ Reverse Transcription System is a kit format and includes GoScript reverse transcriptase, reaction buffer and an optimized set of reagents, including MgCl2, dNTP Mix, Oligo(dT), Random Primers and Recombinant RNasin® Ribonuclease Inhibitor, designed for efficient synthesis of first-strand cDNA. The components of the System can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA.
GoScript™ Reverse Transcription Mix, Oligo(dT) is a convenient mix that includes an optimized set of reagents designed for efficient synthesis of first-strand cDNA. The Oligo(dT) priming initiates first-strand synthesis by annealing to the 3’ end of any polyadenylated RNA molecule. Reverse Transcription of total RNA or mRNA will yield oligo(dT)-primed products from any poly(A)+RNA. The population of cDNAs produced using this method allows many different downstream analyses from a single, reverse transcription reaction.
GoScript™ Reverse Transcription Mix, Random Primers is provided as a user-friendly mix including random primers for synthesis of first-strand cDNA, in preparation for qPCR amplification. Priming using random primers is the most general method of initiating cDNA synthesis from a variety of RNA templates. Random primers can be used to prime first-strand cDNA synthesis from all RNA molecules, including those that do not possess a poly(A)+ tail and RNA isolated from prokaryotic sources.
GoScript™ Reverse Transcriptase contains the GoScript™ M-MLV reverse transcriptase enzyme and the 5X GoScript™ Reactions Buffer.