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The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® RT-qPCR systems.
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GoScript™ Reverse Transcription System
GoScript™ 5X Reaction Buffer
GoScript™ Reverse Transcriptase
PCR Nucleotide Mix
Recombinant RNasin® Ribonuclease Inhibitor
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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For product intended use please see Patents & Disclaimers tab.
Human colon total RNA (Clontech Laboratories, Inc.) was reverse transcribed using oligo(dT) (O) or random primers (R) according to the manufacturer’s instructions for each reverse transcriptase. cDNA then was analyzed by endpoint PCR using GoTaq® Green Master Mix (Cat.# M7122) with primers for a 940bp amplicon at the 5´ end of the transcript. Each reaction (5µl) was analyzed by agarose gel electrophoresis and ethidium bromide staining. All samples shown were analyzed in the same data set and gel with the same scan settings. Gel images are representative of results from three separate experiments.
A5000, A5001 For Research Use Only. Not for Use in Diagnostic Procedures.A5003, A5004 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
A5000, A5001, A5003, A5004 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
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