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Promega Corporation

Tfl DNA PolymerasePCR Satisfaction Guaranteed

Tfl DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. Tfl DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tfl DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures.

Tfl DNA Polymera...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Tfl DNA Polymerase

100u
5u/μl M1941 $ 54.00 Add to cart

Tfl DNA Polymerase

1,000u
5u/μl M1945 $ 454.00 Add to cart


Source

Thermus flavus.

Storage Buffer

20mM Tris-HCl (pH 8.0 at 25°C), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Nonidet®-P40, 0.5% Tween® 20 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTPs into TCA-insoluble material in 30 minutes at 74°C.

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