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Tfl DNA PolymerasePCR Satisfaction Guaranteed

Tfl DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. Tfl DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tfl DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures.

Tfl DNA Polymera...

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Tfl DNA Polymerase

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Tfl DNA Polymerase

 Components

  • Tfl DNA Polymerase

    M194A1 x 100u
  • MgSO4

    M197A1 x 1,250μl
  • Tfl 10X Reaction Buffer

    M200A1 x 750μl
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100u
5u/μl M1941 $ 54.00 Add to cart

Tfl DNA Polymerase

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Tfl DNA Polymerase

 Components

  • Tfl DNA Polymerase

    M194B1 x 1,000u
  • MgSO4

    M197A4 x 1,250μl
  • Tfl 10X Reaction Buffer

    M200B5 x 1.2ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000u
5u/μl M1945 $ 454.00 Add to cart


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Source

Thermus flavus.

Storage Buffer

20mM Tris-HCl (pH 8.0 at 25°C), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Nonidet®-P40, 0.5% Tween® 20 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTPs into TCA-insoluble material in 30 minutes at 74°C.

For product intended use please see Patents & Disclaimers tab.

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Use Restrictions

M1941, M1945 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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