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Experience improved PCR performance with GoTaq® DNA Polymerase products. GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq DNA polymerase. GoTaq® DNA Polymerase comes in a variety of formulations to give you maximum flexibility, control and convenience. GoTaq® Hot Start Polymerase is also available.
GoTaq® Flexi DNA Polymerase allows you to optimize enzyme and magnesium concentration in your PCR. The supplied 5X Green and Colorless Flexi Reaction Buffers do not contain magnesium. A sep...
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GoTaq® Flexi DNA Polymerase allows you to optimize enzyme and magnesium concentration in your PCR. The supplied 5X Green and Colorless Flexi Reaction Buffers do not contain magnesium. A separate tube of 25mM MgCl2 is supplied, giving you maximum control over your reaction conditions. MgCl2 is also available separately.
GoTaq® DNA Polymerase provides improved amplification with the convenience of reaction buffers containing magnesium. The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.
The 5X Green and 5X Colorless Reaction Buffers supplied with GoTaq® enzymes allow you to go directly from thermal cycler to gel analysis. These buffers contain a compound that increases sample density, so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp). Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for post-amplification analysis by fluorescence or absorbance without prior DNA purification.
For ultimate convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix. Both are premixed, ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
For more information, see the Protocols & Applications Guide.
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GoTaq® Flexi DNA Polymerase
5X Colorless GoTaq® Flexi Buffer
5X Green GoTaq® Flexi Buffer
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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GoTaq® DNA Polymerase
5X Green GoTaq® Reaction Buffer
5X Colorless GoTaq® Reaction Buffer
GoTaq® Green Master Mix
GoTaq® Green Master Mix, 2X
GoTaq® Colorless Master Mix
GoTaq® Colorless Master Mix, 2X
Store enzymes at –30°C to –10°C. GoTaq® Green Master Mix can be stored at 4°C for 6 weeks.
For product intended use please see Patents & Disclaimers tab.
Amplification of a 1.2kb fragment of one alpha-1-antitrypsin gene from the indicated number of copies of Human Genomic DNA (Cat.# G3041). Lanes G, GoTaq® Green Master Mix; Lanes N, competitor N; Lanes T, competitor T; Lanes A, competitor A; Lane M, BenchTop 1kb DNA Ladder (Cat.# G7541).
M8291, M8295, M8296, M8297, M8298, M3001, M3005, M3008, M7122, M7123, M7132, M7133 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
M8291, M8295, M8296, M8297, M8298, M3001, M3005, M3008, M7122, M7123, M7132, M7133 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
M8291, M8295, M8296, M8297, M8298, M3001, M3005, M3008, M7122, M7123, M7132, M7133 Use of this product for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.
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