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The Plexor® qPCR and RT-qPCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry. Each target is measured directly during the amplification process and not through a secondary reaction. Plexor® reactions require only two primers for each target.
The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent tag and modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher inserted opposite...
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The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent tag and modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher inserted opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction in fluorescent signal. After PCR, a melt analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting during development. The system also includes a proprietary reagent to minimize primer-dimer formation.
Visit the Plexor® Resources page to learn more.
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.
For more information, see the Protocols & Applications Guide.
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Plexor® qPCR System
Plexor® Master Mix, 2X
Plexor® qPCR Control, 5X
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Plexor® One-Step RT-qPCR System
1.2kb Kanamycin Positive Control RNA
ImProm-II™ Reverse Transcriptase
ImProm-II™ Enzyme Diluent
RNasin® Plus RNase Inhibitor
Plexor® Kanamycin Control Primer Pair, 25X
Plexor® Two-Step RT-qPCR System
ImProm-II™ 5X Reaction Buffer
Store at –20°C.
For product intended use please see Patents & Disclaimers tab.
A4011, A4021, A4051 For Research Use Only. Not for Use in Diagnostic Procedures.
A4011, A4021, A4051 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
A4011, A4021, A4051 The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
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