The Plexor® qPCR and qRT-PCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry. Each target is measured directly during the amplification process and not through a secondary reaction. Plexor® reactions require only two primers for each target. Multiplex assay design is further simplified by the use of the web-based Plexor® Primer Design program specifically engineered for multiplex assay design.
The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent t...
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The Plexor® qPCR and qRT-PCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry. Each target is measured directly during the amplification process and not through a secondary reaction. Plexor® reactions require only two primers for each target. Multiplex assay design is further simplified by the use of the web-based Plexor® Primer Design program specifically engineered for multiplex assay design.
The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent tag and a modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher inserted opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction in the fluorescent signal. After PCR, a melt analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting during development. The system also includes a proprietary reagent to minimize primer-dimer formation.
View an Introduction to the Plexor® Systems animation.
Features - Benefits
- Multiplex Performance: Only two primers are required for each target, making the design of multiplex assays much easier.
- Improve Productivity: Less labor, less time and less cost per assay. Measure controls and targets at the same time in the same well.
- Enjoy Convenience: The master mix format provides everything you need in one tube. Combine with your template and primers.
- Obtain Strong Data: Plexor® Systems measure a reduction in fluorescent signal during amplification. Quenching is directly proportional to amplicon accumulation. After amplification, a melt analysis can be performed to provide an internal control for specificity.
- Use Your Existing Real-Time Instruments: Plexor® technology works on most currently available real-time instruments capable of measuring more than one fluor. The free Plexor® Analysis Software allows users to import and analyze data from their preferred instrument platform. For an up-to-date list of supported instruments, visit the Plexor® Resources page.
- Three Simple Steps to Use Plexor® Systems:
Step 1. Design Your Assay: Choose your primer sets, then order from your preferred oligo provider.
Step 2. Run the Assay: Instruction manuals are available for a wide variety of real-time instruments including Applied Biosystems and Roche. All Plexor® assays are designed for the same cycling conditions.
Step 3. Analyze Your Data: Export the raw data from your real-time instrument, then import into our free Plexor® Analysis Software. The Plexor® software converts the quenching data into cycle threshold (Ct) values and generates standard curves.
Visit the Plexor® Resources page to learn more.
Applications
- qRT-PCR.
- qPCR.
- SNP genotyping.
For more information, see the Protocols & Applications Guide.
References
- Johnson, S.C. et al. (2004) Nucl. Acids Res. 32, 1937–41.
- Frackman, S. et al. (2005) Promega Notes 90, 2–4.
- Sherrill, C.B. et al. (2004) J. Am. Chem. Soc. 126, 4550–6.
- Moser, M.J. et al. (2005) Antimicrob. Agents Chemother. 49, 3334–40.
Patents - Disclaimers
U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
This product is designed and sold for use in the multiplex PCR process covered by U.S. Pat. No. 5,582,989. A limited license has been granted under the patent to use only this amount of the product to practice the multiplex PCR process and is conveyed to the purchaser by the purchase of this product.
The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 5,432,272, 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
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