GoTaq® Real-Time PCR Systems

The GoTaq® qPCR Master Mix is a reagent system for use in real-time quantitative PCR (qPCR and RT-qPCR). The system contains BRYT Green® dye, a proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity. GoTaq® qPCR Master Mix is provided as a premixed, ready-to-use stabilized 2X formulation that includes all components for qPCR....

Expand to Read More »

The GoTaq® qPCR Master Mix is a reagent system for use in real-time quantitative PCR (qPCR and RT-qPCR). The system contains BRYT Green® dye, a proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased reliability, reproducibility and sensitivity. GoTaq® qPCR Master Mix is provided as a premixed, ready-to-use stabilized 2X formulation that includes all components for qPCR.

The GoTaq® 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) protocol. The components and protocol allow robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors, using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix.

The GoTaq® 1-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube. The BRYT Green® Fluorescent Dye and optimized buffer formulations improve data accuracy and sensitivity of low-expressing targets or targets present at low amounts.

Advantages of the GoTaq® Real-Time PCR Systems
The BRYT Green® Fluorescent Dye provides brighter dsDNA-dependent fluorescence than SYBR® Green I. With less PCR inhibition than SYBR® Green I, the dye enables efficient amplification, often resulting in earlier quantification cycle (C_q) values and an expanded linear range using the same filters and settings as SYBR® Green I. The CXR reference dye can be detected using the same filters and settings as those used for ROX™. GoTaq® qPCR Master Mix can be used with any real-time instrument capable of detecting SYBR® Green I or FAM™ dye. GoTaq® qPCR Master Mix contains a low level of CXR reference dye. If your real-time thermal cycler requires a high level of reference dye, add CXR Reference Dye to a final concentration of 1X in the reactions.

Features - Benefits

• Brighter Signal: Sensitive detection for earlier quantitation of low- and high-copy targets.
• Enhanced Stability: Exceptional room-temperature setup makes it suitable for automation and high-throughput detection.
• Versatile: Compatible with both fast and standard qPCR cycling methods.
• Convenient: Read on any instrument capable of reading SYBR® Green I or FAM™ Dye.
• Flexible: Compatible with reverse transcriptase (RT) of choice or a complete solution with 1-step and 2-step systems.
• Robust: High-efficiency, full-length cDNA synthesis in the presence of inhibitors.
• Confidence: The Promega PCR Performance Guarantee.

Applications

• Sensitive sequence detection and quantification (i.e., pathogen detections).
• Gene expression analysis (i.e., validation of microarrays).
• Detection of sequence variants (i.e., SNP genotyping, allele discrimination).
• Fluorescent detection (i.e., DNA/RNA quantification).

Notes

Reaction size is based on a 50µl reaction volume. Fast cycling is suitable using a 20µl reaction volume.

« Collapse Content