TaqBead™ Hot Start Polymerase

TaqBead™ Hot Start Polymerase consists of spherical beads of wax containing Taq DNA polymerase. TaqBead™ Hot Start Polymerase facilitates hot-start PCR by keeping the enzyme sequestered in paraffin wax until the reaction temperature reaches approximately 60°C. This increases PCR specificity by keeping the polymerase separate from the rest of the reaction components until a critical temperature is reached, decreasing the probability of amplifying products that are the result of nonspecific binding of primers. The nonbarrier format of the bead is intended for use with heated-lid thermal cyclers or with the a...

Expand to Read More »

TaqBead™ Hot Start Polymerase consists of spherical beads of wax containing Taq DNA polymerase. TaqBead™ Hot Start Polymerase facilitates hot-start PCR by keeping the enzyme sequestered in paraffin wax until the reaction temperature reaches approximately 60°C. This increases PCR specificity by keeping the polymerase separate from the rest of the reaction components until a critical temperature is reached, decreasing the probability of amplifying products that are the result of nonspecific binding of primers. The nonbarrier format of the bead is intended for use with heated-lid thermal cyclers or with the addition of a mineral oil overlay.

Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0) at 25°C, 1.0% Triton® X-100.

Magnesium Chloride: 25mM MgCl₂ Solution included.

Features - Benefits

• Higher Yield PCR: Hot-start PCR protocols increase amplimer yield by minimizing nonspecific priming, primer-dimer formation or other reactions that can occur at low temperatures once all the PCR amplification components are mixed.
• Increased Specificity: Hot-start format reduces the incidence of nonspecific amplification products.
• Reliable: Minimized nonspecific priming results in increased reproducibility of amplification reactions.
• Flexible: Sufficient 25mM MgCl₂ is provided separately to allow optimization of enzyme performance under different conditions.

« Collapse Content