Promega Corporation

Hot-Start PCR

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Hot-Start PCR Resources

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Hot-Start PCR
Improve PCR Specificity

The proven, robust amplification and sensitivity of GoTaq® DNA Polymerase is now available with a built-in hot start mechanism to deliver even greater specificity.

Featured Products

GoTaq® Hot Start Polymerase

Contains high-performance GoTaq® DNA polymerase bound to a proprietary antibody that blocks polymerase activity below 70°C and is restored during the initial denaturation step, increasing reaction specificity.

GoTaq® Hot Start Master Mixes

Simply add template, water and primers to the ready-to-use solution, and get the specificity and sensitivity of an antibody-based hot-start polymerase in either Green or Colorless Master Mixes for direct-to-gel analysis.

Featured Resources

pubhub_fr Use GoTaq® Hot Start Green and Colorless Master Mixes for Easy, Convenient PCR

In this article we show that GoTaq® Hot Start reactions can sit at room temperature for up to 24 hours before thermal cycling procedure, which can be important for scientists using high-throughput or robotic platforms.

Tools_fr Amplification Product Selector

This selector helps you find the right reagents and systems for your nucleic acid amplification needs.

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