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Promega Corporation

Trypsin/Lys-C Mix, Mass Spec Grade

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.

Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix enhances digestion and compensates for the trypsin proteolytic inefficiency at lysine sites (Figure 1).

Replacing trypsin ...

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Trypsin/Lys-C Mix, Mass Spec Grade

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Trypsin/Lys-C Mix, Mass Spec Grade

 Components

  • Resuspension Buffer

    V181A1 x 500μl
  • Trypsin/Lys-C Mix, Mass Spec Grade

    V507A1 x 20μg
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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Already a Helix Customer?

Request to add this product to your Helix »


20μg
- V5071 $ 51.00 Add to cart

Trypsin/Lys-C Mix, Mass Spec Grade

Close Window

Trypsin/Lys-C Mix, Mass Spec Grade

 Components

  • Resuspension Buffer

    V181A1 x 500μl
  • Trypsin/Lys-C Mix, Mass Spec Grade

    V507B1 x 100μg
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μg
- V5072 $ 128.00 Add to cart

Trypsin/Lys-C Mix, Mass Spec Grade

Close Window

Trypsin/Lys-C Mix, Mass Spec Grade

 Components

  • Resuspension Buffer

    V181A1 x 500μl
  • Trypsin/Lys-C Mix, Mass Spec Grade

    V507A5 x 20μg
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


100μg
(5 × 20µg)
- V5073 $ 138.00 Add to cart


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Storage Conditions

Store Trypsin/Lys-C Mix, Mass Spec Grade, at –30°C to –10°C.

For product intended use please see Patents & Disclaimers tab.

Side-by-side comparison of cleavage sites missed by trypsin or Trypsin/Lys-C Mix using a standard digestion protocol.Figure 1. Side-by-side comparison of cleavage sites missed by trypsin or Trypsin/Lys-C Mix using a standard digestion protocol.
Trypsin/Lys-C Mix provides a critical advantage for protein quantitation.Figure 2. Trypsin/Lys-C Mix provides a critical advantage for protein quantitation.

Incomplete digestion with trypsin was evident after comparing peptide peak intensities in trypsin and Trypsin/Lys-C Mix digests.

Improved mass spec analysis of a poorly purified protein extract.Figure 3. Improved mass spec analysis of a poorly purified protein extract.

The extract contained a residual amount of methanol and chloroform, which inhibits trypsin, but not Trypsin/Lys-C Mix digestion.

Use Restrictions

V5071, V5072, V5073 For Research Use Only. Not for Use in Diagnostic Procedures.

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