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Promega Corporation

Proteases for Mass Spectrometry

Trypsin, the most widely used protease in mass spectrometry analysis, utilizes its high specificity to cleave proteins, resulting in peptides with a strong C-terminal charge. Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin.

When working with tightly folded proteins, the...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart
Arg-C, Sequencing Grade 10μg
- V1881 $ 210.00 Add to cart

Asp-N, Sequencing Grade

2μg
- V1621 $ 168.00 Add to cart

Chymotrypsin, Sequencing Grade

25μg
- V1061 $ 73.00 Add to cart

Chymotrypsin, Sequencing Grade

100μg
(4 × 25μg)
- V1062 $ 218.00 Add to cart
Elastase 5mg
- V1891 $ 68.00 Add to cart

Glu-C, Sequencing Grade

50μg
(5 × 10µg)
- V1651 $ 151.00 Add to cart

Endoproteinase Lys-C, Sequencing Grade

5μg
- V1071 $ 167.00 Add to cart

rLys-C, Mass Spec Grade

15μg
- V1671 $ 120.00 Add to cart
Pepsin 250mg
- V1959 $ 21.00 Add to cart

Sequencing Grade Modified Trypsin

100μg
(5 × 20µg)
- V5111 $ 86.00 Add to cart

Sequencing Grade Modified Trypsin

100μg
(1 × 100µg)
- V5117 $ 77.00 Add to cart

Sequencing Grade Modified Trypsin, Frozen

100μg
(5 × 20µg)
- V5113 $ 43.00 Add to cart
Thermolysin 25mg
- V4001 $ 47.00 Add to cart

Trypsin/Lys-C Mix, Mass Spec Grade

20μg
- V5071 $ 51.00 Add to cart

Trypsin/Lys-C Mix, Mass Spec Grade

100μg
- V5072 $ 128.00 Add to cart

Trypsin/Lys-C Mix, Mass Spec Grade

100μg
(5 × 20µg)
- V5073 $ 138.00 Add to cart

Trypsin Gold, Mass Spectrometry Grade

100μg
- V5280 $ 108.00 Add to cart


Storage Conditions

Store Cat.# V1881, V1621, V1061, V1062, V1891, V1651, V1071 and V1959 at 2–10°C.

Store Cat.# V1671, V5111, V5117, V4001, V5071, V5072, V5073 and V5280 at –30°C to –10°C.

Store Cat.# V5113 at –70°C.

For product intended use please see Patents & Disclaimers tab.

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