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Promega Corporation

Multimode Readers Comparison Guide

Untitled 1
  GloMax-Multi Jr Single Tube GloMax-Multi+ Microplate with Instinct Software GloMax-Multi Jr Single Tube
  GloMax® Discover
Multimode Detection System
GloMax®-Multi+ Microplate
with Instinct® Software
GloMax®-Multi Jr
Single Tube
 
Product Overview Request Pricing »More Information » Request Pricing »More Information » Request Pricing »More Information »
Catalog No. GM3000 E8032 with Shaking
E9032 with Heating and Shaking
E6070 Base Instrument
E6080 Instrument with Luminescence Installed
Product Type 6- to 384-well Microplate Multimode Reader 6-384-well Microplate Multimode Reader Single Tube Multimode Reader
Description High-performance microplate reader with expanded detection modes, easy-to-use software, and integration capability for automated workflows. Versatile Microplate Reader with Shaking, Instinct® Software, and Optional Heating.
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Multifunctional single tube instrument for utmost flexibility.
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Available Detection Modes Luminescence, Filtered Luminescence, Fluorescence Intensity, BRET, FRET, and UV-Visible Absorbance Luminescence:
E8041 Luminescence
Fluorescence:
E8051 Fluorescence
Absorbance:
E8061 Visible
E9061 UV Visible
Luminescence:
E6098 Luminescence (or installed in instrument E6080)
Fluorescence:
E6071 Blue
E6072 UV
E6073 Green
E6074 Red
E6075 GFPUV
Absorbance:
E6076 Absorbance Module. Add 1 or more filter paddle(s):
E6077 560nm Filter
E6078 600nm Filter
E6079 750nm Filter
Read Type Glow, Flash, Kinetic, Repeat Glow, Flash, Kinetic, Repeat Glow, Kinetic, Repeat
Sample Format 6-, 12-, 24-, 48-, 96- and 384-well plates 6, 12, 24, 48, 96, 384-well plates 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional), 0.5 ml PCR tube (optional, fluorescence), 1.5 ml microcentrifuge tube (Luminescence)
Shaking Linear or orbital patterns Four speeds (150, 300, 500 rpm, Variable) linear and orbital modes Not Available
Temperature Control Ambient + 5°C to 45°C Optional: 2° C above ambient temperature to 45° C +/- 0.75° C Not Available
Injectors Optional: Two Injectors Optional:
E7071 Single Injector
E7081 Dual Injectors

Selectable between 25 - 200 µl in 5 µl increments for 6 to 96-well plate formats.
Not Available
Luminescence Light Standard Optional: Luminescence Light Plate
Luminescence      
Detector Top-reading, head-on photon counting photomultiplier tube (PMT) Head-on photomultiplier tube (PMT) for photon-counting Photomultiplier tube (PMT)
Detection Limit 3 × 10-21 moles of luciferase (96-well plate) 3 x 10-21 moles of Luciferase or 1 x 10-18 moles of ATP 1 x 10-16 moles ATP or 1 x 10-20 moles of Luciferase
Linear Dynamic Range 9 logs (assay dependent) > 8 logs 5 logs
Crosstalk Less than 3 × 10-5 (white 96-well plate) 5 x 10-5 Not Applicable
Sample Adapter Not Applicable Not Applicable Optional. Holds 1.5 ml microcentrifuge tubes
Spectral Range 350–650 nm 350 - 650 nm 350 - 650 nm
Peak Wavelength Filter paddle containing 4 standard filters for luminescence including: 495nm SP, 530nm LP, 540nm SP, 600nm LP, and an empty, customizable position. Additional custom emission filters may also be used. Contact Promega Technical Services. 420 nm 420 nm
Fluorescence      
Light Source Wavelength-matched LED Wavelength-matched LED Wavelength-matched LED
Detector PiN photodiode PIN-photodiode Photodiode
Read Position Top-reading Top reading 90 degree
Wavelength Selection Built-in and customizable filter paddle Snap-in Fluorescence Optical Kits Snap-in Fluorescence Modules (user installable)
Wavelengths UV (Ex: 365nm, Em:414–445nm)

Blue (Ex:475nm, Em: 500–550nm)

Green (Ex: 525nm, Em: 580–640nm)

Red (Ex: 625nm, Em: 660–720nm)

AFC (Ex: 405nm, Em: 495–505nm)
UV (Ex 365 nm: Em 410 - 460 nm)

Blue (Ex 490 nm: Em 510 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 720 nm)

AFC (Ex 405 nm: EM 495 - 505 nm)
UV (Ex 365 nm: Em 410 - 450 nm)

Blue (Ex 460 nm: Em 515 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 725 nm)

UV-GFP (Ex: 365 nm, Em: 515 - 570 nm)
Detection Limit 2fmol/200µl fluorescein in a 96-well plate 0.5 fmol/200 µl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA using DNA Quantation Dye 6 ppt fluorescein, 450 pg/ml DNA using DNA Quantation Dye
Linear Dynamic Range 6 logs (assay-dependent) 6 logs, assay dependent 6 logs, assay dependent
Read Out Relative Fluorescent Units (RFU) Relative Fluorescence Units, Direct Concentration Fluorescence Standard Units, Direct concentration
Calibration Not Applicable   1 to 5 point calibration
Discrete Sample Averaging Sample readings are averaged over duration of the run, specified by user Sample readings are averaged over 0.5 second to improve accuracy Sample readings are averaged over 5 seconds to improve accuracy
Visible Absorbance      
Light Source Xenon flash lamp LED LED
Detector Top-reading, head-on photon counting photomultiplier tube (PMT) Large-area photodiode Photodiode
Spectral Range 200-600 nm 450 - 750 nm 400 - 800 nm
Wavelengths for Installed Filters Filter wheel with 9 absorbance filters including: 230, 260, 280, 320, 405, 450, 490, 560, and 600nm 10mm band pass 450, 560, 600, 750 nm, 2 slots for customization 560 nm, 600 nm, 750 nm
Photometric Measuring Range 0.1 O.D. 0 - 5.0 OD 0 - 4.0 OD
Linear Dynamic Range 0-4.0 O.D. at 260nm and 280nm 0 - 4.0 OD, assay dependent 0 - 2.5 OD, assay dependent
OD Accuracy Not Applicable 0.01 OD ± 3% at ≤ 2.5 OD ±0.7%
OD Precision Not Applicable 0.01 OD ± 1% ±0.5% at 1.0 OD
Stray Light Not Applicable 0.002% at 560 nm in clear bottom, black wall plate Not Applicable
UV-Visible Absorbance      
Light Source Xenon flash lamp Xenon Lamp Not Applicable
Detector Top-reading, head-on photon counting photomultiplier tube (PMT) Photodiode Not Applicable
Spectral Range 200-600nm 200 - 1100 nm Not Applicable
Wavelengths for Installed Filters Filter wheel with 9 absorbance filters including: 230, 260, 280, 320, 405, 450, 490, 560, and 600nm 10mm band pass 260, 280, 450, 560, 600, 750 nm (2 slots customizable) Filter wheel with 9 absorbance filters including: 230, 260, 280, 320, 405, 450, 490, 560, and 600nm 10mm band pass
Photometric Measuring Range 0.1 O.D. 0 - 4.0 OD Not Applicable
Linear Dynamic Range 0-4.0 O.D. at 260nm and 280nm 0 - 3.5 OD, assay dependent Not Applicable
OD Accuracy Not Applicable 0.05 OD ± 3% at ≤ 2.5 OD Not Applicable
OD Precision Not Applicable 0.002 ± 3% Not Applicable
Stray Light Not Applicable 0.001% at 560 nm in clear bottom, black wall plate Not Applicable
Instrument      
User Interface Windows®8 Tablet PC (included) with USB connection to instrument Built-in PC, touch screen navigation and operation Touch screen navigation and operation
Data Output Wireless connectivity to wireless network, USB Flash drive, microSD card, or cloud location PC compatible ASCII text file format exported to USB flash drive, or connect to PC (not included) through USB or Serial Port Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available
External PC Requirements Windows® 8, 64 bit, with USB connection to instrument Windows XP or higher Windows XP SP2 or later
Computer Interface USB type B USB type B or RS-232 port 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud
Power 100-240 VAC, 50/60 Hz. 100 - 240 VAC, 50 - 60 Hz Instrument: 12V DC, 0.7A Max, AC Adapter: 100-240V AC, 50/60Hz, 0.3A
Auto-Shutoff None Touch screen hibernates after 15 minutes of inactivity Touch screen hibernates after 15 minutes of inactivity
Information      
Application Notes View Application Notes View Application Notes View Application Notes
Frequently Asked Questions Read Frequently Asked Questions Read Frequently Asked Questions
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