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Promega Corporation

Fluorometer Comparison Guide

  GloMax-Multi+ Microplate with Instinct Software GloMax-Multi Jr Single Tube Quantus Fluorometer
  GloMax®-Multi+ Microplate
with Instinct® Software
GloMax®-Multi Jr
Single Tube
Quantus™ Fluorometer
 
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Catalog No. E8032 with Shaking
E9032 with Heating and Shaking
E6070 Base Instrument
E6080 Instrument with Luminescence Installed
E6150
Product Type 6-384-well Microplate Multimode Reader Single Tube Multimode Reader Single Tube Fluorometer
Description Versatile Microplate Reader with Shaking, Instinct® Software, and Optional Heating.
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Multifunctional single tube instrument for utmost flexibility.
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Compact, easy to use, low cost fluorometer.
Available Detection Modes Luminescence:
E8041 Luminescence
Fluorescence:
E8051 Fluorescence
Absorbance:
E8061 Visible
E9061 UV Visible
Luminescence:
E6098 Luminescence (or installed in instrument E6080)
Fluorescence:
E6071 Blue
E6072 UV
E6073 Green
E6074 Red
E6075 GFPUV
Absorbance:
E6076 Absorbance Module. Add 1 or more filter paddle(s):
E6077 560nm Filter
E6078 600nm Filter
E6079 750nm Filter
Fluorescence
Read Type Glow, Flash, Kinetic, Repeat Glow, Kinetic, Repeat Discrete
Sample Format 6, 12, 24, 48, 96, 384-well plates 10 x 10 mm cuvette, 100 - 200 µl minicell cuvette (optional), 0.5 mL PCR tube (100 µl minimum, optional, fluorescence) or 1.5 ml microcentrifuge tube (Luminescence) 0.5 mL PCR tube
Shaking Four speeds (150, 300, 500 rpm, Variable) linear and orbital modes Not Applicable Not Applicable
Temperature Control Optional: 2° C above ambient temperature to 45° C +/- 0.75° C Not Applicable Not Applicable
Dispense Optional:
E7071 Single Injector
E7081 Dual Injectors

Selectable between 25 - 200 µl in 5 µl increments for 6 to 96-well plate formats.
Not Applicable Not Applicable
Fluorescence      
Light Source Wavelength-matched LED Wavelength-matched LED Wavelength-matched LED
Detector PIN-photodiode Photodiode Solid state silicon sensor
Read Position Top reading Not Applicable Not Applicable
Wavelength Selection Snap-in Fluorescence Optical Kits Snap-in Fluorescence Modules 2 modes
Wavelengths UV (Ex 365 nm: Em 410 - 460 nm)

Blue (Ex 490 nm: Em 510 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 720 nm)

AFC (Ex 405 nm: EM 495 - 505 nm)
UV (Ex 365 nm: Em 410 - 450 nm)

Blue (Ex 460 nm: Em 515 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 725 nm)

UV-GFP (Ex: 365 nm, Em: 515 - 570 nm
Blue (Ex 495 nm, Em 510 - 580 nm)

Red (Ex 640 nm, Em 660 – 720 nm)
Detection Limit 0.5 fmol/200 µl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA 6 ppt fluorescein, 450 pg/ml DNA 1 pg/ml dsDNA, 3.9 pg/ml RNA
Linear Dynamic Range 6 logs, assay dependent 6 logs, assay dependent Five (5) orders of magnitude, assay dependent
Read Out Relative Fluorescence Units, Direct Concentration Fluorescence Standard Units, Direct concentration Direct sample concentration and Raw value readouts; flexible user inputs for sample volume and concentration units
Calibration Multi-point calibration 1 - 5 point calibration Single-point calibration
Discrete Sample Averaging Sample readings are averaged over 0.5 second to improve accuracy Sample readings are averaged over 5 seconds to improve accuracy Five sample readings are averaged over a 0.5 second period to improve accuracy
Instrument      
User Interface Built-in PC, touch screen navigation and operation Touch screen navigation and operation Menu-driven keypad and LCD display
Data Output PC compatible ASCII text file format exported to USB flash drive, or connect to PC (not included) through USB or Serial Port Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available. The Quantus™ Fluorometer will save the last 20 measurements. Results can be transferred to a personal computer using Quantus™ Software, and exported to Microsoft® Excel.
External PC Requirements Optional. Windows XP or Vista Optional. Windows XP SP2 or higher For Optional Quantus™ Software: Windows® 8, Windows® 7 (32 or 64 bit) .NET Framework 4.0 or later; At least one USB port available
Computer Interface USB type B or RS-232 port 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud USB port
Power 100 - 240 VAC, 50 - 60 Hz Instrument: 12V DC, 0.7A Max, AC Adapter: 100-240V AC, 50/60Hz, 0.3A SV, 0.2A Maximum
Auto-Shutoff Touch screen hibernates after 15 minutes of inactivity Touch screen hibernates after 15 minutes of inactivity Sleep Mode after 1 hour of inactivity
Alarms Not Applicable Not Applicable Not Applicable
Information      
Application Notes View Application Notes View Application Notes View Application Notes
Frequently Asked Questions Read Frequently Asked Questions Read Frequently Asked Questions Read Frequently Asked Questions
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