VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) is a firefly luciferase prosubstrate containing the DEVD tetrapeptide sequence recognized by caspase-3 and -7. Upon activation of caspase-3 or -7, the DEVD peptide is cleaved, and the liberated aminoluciferin reacts with luciferase to generate measurable light. Cleavage has been shown in in cellulo and in vivo systems. For mice, activity of a related salt was demonstrated when 10mg of the substrate in 150μl of saline was injected intraperitoneally. Other references suggest that doses as low as 1.5mg per mouse (50mg/kg) can be used. We recommend co...
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VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) is a firefly luciferase prosubstrate containing the DEVD tetrapeptide sequence recognized by caspase-3 and -7. Upon activation of caspase-3 or -7, the DEVD peptide is cleaved, and the liberated aminoluciferin reacts with luciferase to generate measurable light. Cleavage has been shown in in cellulo and in vivo systems. For mice, activity of a related salt was demonstrated when 10mg of the substrate in 150μl of saline was injected intraperitoneally. Other references suggest that doses as low as 1.5mg per mouse (50mg/kg) can be used. We recommend conducting a preliminary dose-response study using no more than 500mg/kg.
VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) has a minimum solubility of 500mg/ml in PBS, and the resulting solution is stable for at least 3 days at room temperature. Injection is usually done via the intraperitoneal route, and imaging is generally started 10 minutes after injection.
VivoGlo™ In Vivo Imaging Substrates are provided in cooperation with Xenogen Corporation and Caliper Life Sciences for use in in vivo bioluminescence imaging applications.
Features - Benefits
- Highest Quality Substrates: Eliminate potential interference in assays due to the presence of endotoxins.
- Assured Product Integrity: Most products are packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments. Product is packaged with fine tolerances to minimize the need to weigh substrates.
- Flexibility and Convenience: Available in multiple sizes to accommodate a variety of experimental settings.
References
- Liu, J.J. et al. (2005) Cancer Biol. Ther. 4(8), 885 92.
- Shah, K. et al. (2005) Mol. Ther. 11(6), 926 31.
- Kizaka-Kondoh, S. et al. (2009) Clin. Cancer Res. 15, 3433 41.
- Scabini, M. In vivo imaging of early stage apoptosis measuring real-time caspase-3 activation. Presentation at: 4th European Molecular Imaging Meeting; 2009 May 27 30, Barcelona, Spain.
Patents - Disclaimers
The use of this product and derivatives thereof is strictly limited to that of a life sciences research reagent. All other use is strictly prohibited, including but not limited to any diagnostic, therapeutic, or commercial use. The use of this product for in vivo bioluminescent imaging may be covered by one or more patents controlled by Caliper Life Sciences, Inc. Those patents include U.S. Pat. Nos. 5,650,135, 6,217,847, 6,923,951, 6,908,605, 6,890,515, and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Caliper Life Sciences, Inc., 68 Elm St., Hopkinton, MA 01748 for information regarding a license to practice under such patents.
This product does not convey a license to use recombinant Coleoptera luciferase under U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Promega sells licensed Coleoptera luciferase vectors, which may be used in conjunction with this product.
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