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Promega Corporation

Horseradish Peroxidase-Conjugated Antibodies

Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme. The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The products (unless otherwise noted) are supplied as 1mg/ml solutions. A starting working dilution of 1:2,500 is suggested for...

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Anti-Rabbit IgG (H+L), HRP Conjugate

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Anti-Rabbit IgG (H+L), HRP Conjugate

 Components

  • Anti-Rabbit IgG HRP Conjugate

    W401B1 x 300μl
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  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


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300μl
- W4011 $ 104.00 Add to cart

Anti-Mouse IgG (H+L), HRP Conjugate

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Anti-Mouse IgG (H+L), HRP Conjugate

 Components

  • Anti-Mouse IgG HRP Conjugate

    W402B1 x 300μl
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  • This product can be added to a

  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


300μl
- W4021 $ 104.00 Add to cart

Anti-Human IgG (H+L), HRP Conjugate

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Anti-Human IgG (H+L), HRP Conjugate

 Components

  • Anti-Human IgG HRP Conjugate

    W403B1 x 300μl
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  • This product can be added to a

  • Helix Refrigerator

This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


300μl
- W4031 $ 104.00 Add to cart

Anti-Chicken IgY, HRP Conjugate

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Anti-Chicken IgY, HRP Conjugate

 Components

  • Anti-Chicken IgY, HRP Conjugate

    G135A1 x 300μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


300μl
- G1351 $ 138.00 Add to cart

Donkey Anti-Goat IgG, HRP

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Donkey Anti-Goat IgG, HRP

 Components

  • Donkey Anti-Goat IgG HRP

    V805A1 x 60μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


60μl
- V8051 $ 121.00 Add to cart


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Storage Conditions

Store the unopened product at –20°C. Store opened Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate at 4°C.

For product intended use please see Patents & Disclaimers tab.

Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.Figure 1. Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of total protein (µg). Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and 1-minute exposure.

Enzyme-linked immunosorbent assay (ELISA) for β-actin.Figure 2. Enzyme-linked immunosorbent assay (ELISA) for β-actin.

Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; ELISA developed with TMB One Solution (Cat.# G7431).

Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; ELISA developed with TMB One Solution (Cat.# G7431).

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Use Restrictions

W4011, W4021, W4031, G1351, V8051 For Research Use Only. Not for Use in Diagnostic Procedures.

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