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The PowerPlex® 16 BIO System is a multiplex STR system for use in DNA typing, including paternity testing, forensic DNA analysis, human identity testing and tissue culture strain identification. The system allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin): Penta E, D18S51, D21S11, TH01, D3S1358 (labeled with fluorescein); FGA, TPOX, D8S1179, vWA, Amelogenin (labeled with Rhodamine Red™-X); and Penta D, CSF1PO, D16S539, D7S820, D13S317, D5S818 (labeled with JOE). The amplified STRs are detected using the Hitachi FMBIO® II Fluorescence Imaging System.
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High-throughput analysis is achieved by using the available software to compare amplified DNA fragments directly with the allelic ladder provided for each locus. The allelic ladder mixture consists of most or all known alleles in the population and is included to allow rapid and precise assignment of alleles. The size marker (Internal Lane Standard 600 BIO) provided with the system is tagged with a fourth fluorescent dye. This marker is included as a standard to increase precision in analysis when using the PowerPlex® 16 BIO System.
The Matrix 16 BIO is provided to help properly generate a color matrix for color separation using a filter set* and the Hitachi FMBIO® Analysis Software. The Matrix 16 BIO consists of fragments labeled with four different fluorescent dyes: Fluorescein, JOE, Rhodamine Red™-X and Texas Red®-X. These four dyes are the same as those used in the PowerPlex® 16 BIO System.
*Filter sets (MiraiBio Cat.# 11999-246-00) are available for purchase from MiraiBio, Inc., at 800-624-6176 (USA) or 510-337-2000.
For more information, see the Protocols & Applications Guide.
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PowerPlex® 16 BIO System
2800M Control DNA
PowerPlex® 16 BIO Allelic Ladder Mix
Internal Lane Standard 600 BIO
Matrix 16 BIO
PowerPlex® 16 BIO 10X Primer Pair Mix
Gold ST★R 10X Buffer
Gel Tracking Dye
Bromophenol Blue Loading Solution
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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This product is available through the Promega Helix onsite stocking program in a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs. The Helix Refrigerator is 5 cubic ft.
Store at –20°C. The fluorescent primer pairs and allelic ladders are light-sensitive; therefore, minimize light exposure.
For product intended use please see Patents & Disclaimers tab.
DC6540, DD7101, DD7251, DD1001 Not For Medical Diagnostic Use.
DC6540 U.S. Pat. No. 6,238,863 and other patents and patents pending.
DC6540 Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.
DC6540 PowerPlex® 16 BIO System incorporates dye conjugates made with the Rhodamine Red™-X and Texas Red®-X fluorescent reactive dyes, which are licensed from Molecular Probes, Inc., under U.S. Pat. Nos. 5,798,276 and 5,846,737 for DNA analysis. Rhodamine Red is a trademark of Molecular Probes, Inc., and Texas Red is a registered trademark of Molecular Probes, Inc.
DC6540 The purchase of this product does not convey a license to use AmpliTaq Gold® DNA polymerase. You should purchase AmpliTaq Gold® DNA polymerase licensed for the forensic and human identity field directly from your authorized enzyme supplier.
DC6540 Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.
DC6540 STR loci are the subject of U.S. Pat. No. RE 37,984, German Pat. No. DE 38 34 636 C2 and other patents issued to the Max-Planck-Gesellschaft zur Förderung der Wissenschaften, e.V., Germany.
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