PowerPlex® Y23 System

More Ys in Half the Time... See Y

The PowerPlex® Y23 System allows Y-STR analysis of human forensic and database samples. The 5-dye STR multiplex amplifies 23 Y-STR loci, including two rapidly mutating Y-STR loci.

The system enables laboratories to:

  • Detect more male DNA in the presence of excess female DNA
  • Obtain a full profile from challenging casework samples, even those containing high concentrations of inhibitors
  • Save time in every run with rapid-cycling protocols
  • Simplify workflows with one kit for both casework and database samples

Amplify 23 Y-STR Loci in a Single Amplification Reaction

The inclusion of six highly informative loci can help improve a laboratory's ability to distinguish individuals from different parental lines, providing more meaningful analyses and making the system ideal for:

  • Forensic casework on sexual assault evidence
    • High female-to-male DNA ratio
    • Multiple male profiles
  • Familial searching
  • Paternity testing
  • Missing persons investigations
  • Migration and evolutionary studies
  • Historical and genealogical research
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The PowerPlex® Y23 System allows co-amplification and four-color detection of 23 male-specific STR loci: DYS576, DYS389I/II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438 (penta), DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643 (penta), DYS393, DYS458, DYS385a/b, DYS456 and Y-GATA-H4.
10436MB_1320x663
The PowerPlex® Y23 System allows co-amplification and four-color detection of 23 male-specific STR loci: DYS576, DYS389I/II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438 (penta), DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643 (penta), DYS393, DYS458, DYS385a/b, DYS456 and Y-GATA-H4.

Amplify Only Male DNA

The PowerPlex® Y23 System can successfully amplify trace amounts of male DNA, even in the presence of overabundant amounts of female DNA.

13065MA
Amplification of decreasing amounts of male DNA in the presence of 1µg of female DNA with two Y-STR kits: PowerPlex® Y23 System and Yfiler® Plus PCR Amplification Kit. Amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).
13065MA
Amplification of decreasing amounts of male DNA in the presence of 1µg of female DNA with two Y-STR kits: PowerPlex® Y23 System and Yfiler® Plus PCR Amplification Kit. Amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).

Obtain Full Profiles from the Most Challenging Samples

The PowerPlex® Y23 System is a robust multiplex that is tolerant of many amplification inhibitors, including hematin, tannic acid and humic acid. The robust performance of the kit results in more interpretable data and less need for re-amplification of inhibitor-laden samples.

13066MA
Amplification of 500pg of male DNA (PowerPlex® Y23 System) or 1.0ng of male DNA (Yfiler® Plus PCR Amplification Kit) in the presence of increasing amounts of common inhibitors. DNA was amplified for 30 cycles, and amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).
13066MA
Amplification of 500pg of male DNA (PowerPlex® Y23 System) or 1.0ng of male DNA (Yfiler® Plus PCR Amplification Kit) in the presence of increasing amounts of common inhibitors. DNA was amplified for 30 cycles, and amplified products were separated on an Applied Biosystems® 3500xL Genetic Analyzer (1.2kV, 24-second injection).

Streamline Workflows with Direct Amplification

The PowerPlex® Y23 System also is compatible with direct amplification, helping to streamline Y-STR databasing efforts. Amplification can be successfully performed with sample types such as FTA® card punches as well as pretreated swabs, Bode Buccal DNA Collector™ punches or S&S 903 punches.

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Amplification of DNA from two 1.2mm punches from buccal cells on an FTA® card (direct amplification) using 27 cycles and the PowerPlex® Y23 System. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
10433MA_1320x899
Amplification of DNA from two 1.2mm punches from buccal cells on an FTA® card (direct amplification) using 27 cycles and the PowerPlex® Y23 System. Amplified products were separated using an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
13067MA
Amplification of DNA from a cotton swab pretreated with SwabSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
13067MA
Amplification of DNA from a cotton swab pretreated with SwabSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
13068MA
Amplification of one 1.2mm Bode Buccal DNA Collector™ punch preprocessed with the PunchSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
13068MA
Amplification of one 1.2mm Bode Buccal DNA Collector™ punch preprocessed with the PunchSolution™ Reagent using 26 cycles and the PowerPlex® Y23 System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).