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The Proteasome-Glo™ Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells. Adding the Proteasome-Glo™ Cell-Based Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.
The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminol...
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The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminoluciferin for chymotrypsin-like, Z-LRR-aminoluciferin for trypsin-like, and Z-nLPnLD-aminoluciferin for caspase-like activity. Each luminogenic substrate is added to a buffer system optimized for its specific proteasome activity and luciferase activity. The reagents are added to test samples containing proteasome enzyme that cleaves the substrates, releasing luciferin, which is consumed by luciferase, producing "glow-type" luminescence correlating to enzyme activity or inhibition.
The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays in an enzyme-based format (each of these three assays also is available separately).
The Proteasome-Glo™ Cell-Based 3-Substrate System consists of three homogeneous bioluminescent assays that measure the three proteolytic activities associated with the proteasome in a cell-based format (each of these three assays also is available separately).
Cat.# G8621, G8631, G8641 and G8531 (for each of the 3 systems) are each sufficient for 100 assays at 100μl/assay in 96-well plates or 400 assays at 25μl/assay in 384-well plates. Cat.# G8622, G8632, G8642 and G8532 (for each of the 3 systems) are each sufficient for 500 assays at 100μl/assay in 96-well plates or 2,000 assays at 25μl/assay in 384-well plates.
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Proteasome-Glo™ Chymotrypsin-Like Assay
Luciferin Detection Reagent
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Proteasome-Glo™ Trypsin-Like Assay
Proteasome-Glo™ Caspase-Like Assay
Proteasome-Glo™ 3-Substrate System
Store the Proteasome-Glo™ Assay components at –20°C.
For product intended use please see Patents & Disclaimers tab.
G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 For Research Use Only. Not for Use in Diagnostic Procedures.
G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 Patent Pending.
G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.
G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
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