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Promega Corporation

Protease-Glo™ Assay

The Protease-Glo™ Assay is a novel method to detect and measure protease activities using a genetically engineered firefly (Photinus pyralis) luciferase and represents one example of the GloSensor™ platform technology. The assay uses a circularly permuted firefly luciferase, the GloSensor™-10F protein, with a protease recognition site as the protease substrate. This assay system allows rapid generation of protease substrates through molecular cloning and coupled transcription/translation cell-free expression, thus enabling the facile evaluation of protease function. Oligonucleotides encoding a protease recognition...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Protease-Glo™ Assay

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Protease-Glo™ Assay

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • Bright-Glo™ Luciferase Assay Substrate

    E263A1 x 1 vial
  • Bright-Glo™ Luciferase Assay Buffer

    E264A1 x 10ml
  • pGloSensor™-10F Linear Vector

    G946A1 x 20μl
  • pGloSensor™-10F(TEV) Control Plasmid

    G947A1 x 100μl
  • TnT® SP6 High-Yield Wheat Germ Master Mix

    L326A1 x 300μl
  • T4 DNA Ligase

    M180A1 x 100u
  • DTT

    P117B1 x 250μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • ProTEV Buffer, 20X

    V602A1 x 1ml
  • ProTEV Plus

    V610A1 x 1,000u
1 each
- G9451 $ 1,031.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

pGloSensor™-10F Linear Vector

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pGloSensor™-10F Linear Vector

  Components

  • pGloSensor™-10F Linear Vector

    G946A1 x 20μl
1μg
- G9461 $ 619.00 Add to cart

GenBank®/EMBL Accession Number

pGloSensor™ -10F Linear Vector, EU693492; pGloSensor™ -10F Linear Vector Sequence (.txt file)
pGloSensor™ -10F[TEV] Control Plasmid, EU693493; pGloSensor™ -10F[TEV] Control Plasmid Sequence (.txt file)

Storage Conditions

Store all components at –20°C, except the TnT® SP6 High-Yield Wheat Germ Master Mix, which must be stored at –70°C.

For product intended use please see Patents & Disclaimers tab.

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