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Promega Corporation

DUB-Glo™ Protease Assay

The DUB-Glo™ Protease Assay (DUB/SENP/NEDP) is a homogeneous, bioluminescent assay that measures the activity of numerous deconjugating enzymes including deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases. These proteases reverse the protein modification by ubiquitin and ubiquitin-like proteins (Ubl proteins) and thus are integral components in the complex mechanisms of posttranslational protein regulation in eukaryotes.

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DUB-Glo™ Protease Assay (DUB/SENP/NEDP)

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DUB-Glo™ Protease Assay (DUB/SENP/NEDP)

 Components

  • DUB-Glo™ Buffer

    G624A1 x 10ml
  • Z-RLRGG-Glo™ Substrate

    G625A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G6260 $ 406.00 Add to cart

DUB-Glo™ Protease Assay (DUB/SENP/NEDP)

Close Window

DUB-Glo™ Protease Assay (DUB/SENP/NEDP)

 Components

  • DUB-Glo™ Buffer

    G624B1 x 50ml
  • Z-RLRGG-Glo™ Substrate

    G625B1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G6261 $ 1,282.00 Add to cart


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Storage Conditions

Store components at –20°C protected from light.

For product intended use please see Patents & Disclaimers tab.

The luminogenic substrate, Z-RLRGG-aminoluciferin, is recognized by numerous deconjugating proteases including DUBs, SENPs and NEDP1.Figure 1. The luminogenic substrate, Z-RLRGG-aminoluciferin, is recognized by numerous deconjugating proteases including DUBs, SENPs and NEDP1.

Following cleavage by the protease, the substrate for luciferase (aminoluciferin) is released, allowing the luciferase reaction to produce light.

A comparison of the DUB-Glo Protease Assay (DUB/SENP/NEDP) to fluorescent assays for UCH-L3.Figure 2. A comparison of the DUB-Glo™ Protease Assay (DUB/SENP/NEDP) to fluorescent assays for UCH-L3.

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Use Restrictions

G6260, G6261 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G6260, G6261 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G6260, G6261 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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