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The DUB-Glo™ Protease Assay (DUB/SENP/NEDP) is a homogeneous, bioluminescent assay that measures the activity of numerous deconjugating enzymes including deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases. These proteases reverse the protein modification by ubiquitin and ubiquitin-like proteins (Ubl proteins) and thus are integral components in the complex mechanisms of posttranslational protein regulation in eukaryotes.
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Cat.# G6260 is sufficient for 100 assays at 100μl/assay or 200 assays at 50μl/assay in 96-well plates, or 400 assays at 25μl/assay in 384-well plates. Cat.# G6261 is sufficient for 500 assays at 100μl/assay or 1,000 assays at 50μl/assay in 96-well plates, or 2,000 assays at 25μl/assay in 384-well plates.
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DUB-Glo™ Protease Assay (DUB/SENP/NEDP)
Luciferin Detection Reagent
Store components at –20°C protected from light.
For product intended use please see Patents & Disclaimers tab.
Following cleavage by the protease, the substrate for luciferase (aminoluciferin) is released, allowing the luciferase reaction to produce light.
G6260, G6261 For Research Use Only. Not for Use in Diagnostic Procedures.
G6260, G6261 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.
G6260, G6261 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
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