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Promega Corporation

DPPIV-Glo™ Protease Assay

The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. The DPPIV-Glo™ Assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a single DPPIV-Glo™ Reagent in an "add-mix-measure" format results in DPPIV cleavage of the substrate and generation of a "glow-type" luminescent signal produced by the luciferase reaction. In...

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DPPIV-Glo™ Protease Assay

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DPPIV-Glo™ Protease Assay

 Components

  • DPPIV-Glo™ Buffer

    G836A1 x 10ml
  • DPPIV-Glo™ Substrate

    G837A1 x 26.5μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8350 $ 309.00 Add to cart

DPPIV-Glo™ Protease Assay

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DPPIV-Glo™ Protease Assay

 Components

  • DPPIV-Glo™ Buffer

    G836B1 x 50ml
  • DPPIV-Glo™ Substrate

    G837B1 x 115.2μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G8351 $ 802.00 Add to cart


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Storage Conditions

Store components at –20°C protected from light.

For product intended use please see Patents & Disclaimers tab.

The proluminescent substrate containing the Gly-Pro sequence recognized by DPPIV.Figure 1. The proluminescent substrate containing the Gly-Pro sequence recognized by DPPIV.

Following DPPIV cleavage, the substrate for luciferase (aminoluciferin) is released, resulting in the luciferase reaction and the production of light.

Overview of the preparation and use of the DPPIV-Glo Reagent.Figure 2. Overview of the preparation and use of the DPPIV-Glo™ Reagent.
Sensitivity of the DPPIV-Glo Protease Assay compared to a fluorescent assay.Figure 3. Sensitivity of the DPPIV-Glo™ Protease Assay compared to a fluorescent assay.

Use Restrictions

G8350, G8351 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8350, G8351 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G8350, G8351 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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