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Promega Corporation

Cell-Based Proteasome-Glo™ Assays

The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells. Proteasome-Glo™ Cell-Based Assays provide luminogenic proteasome substrates in buffers optimized for cell permeabilization, proteasome activity and luciferase activity. Addition of the Proteasome-Glo™ Cell-Based Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapi...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

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Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

 Components

  • Suc-LLVY-Glo™ Substrate

    G856C1 x 50μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865A1 x 10ml
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8660 $ 453.00 Add to cart

Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

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Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

 Components

  • Suc-LLVY-Glo™ Substrate

    G856C5 x 50μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865A5 x 10ml
  • Luciferin Detection Reagent

    V859A5 x 1 each
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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5 × 10ml
- G8661 $ 1,418.00 Add to cart

Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

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Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

 Components

  • Suc-LLVY-Glo™ Substrate

    G856D2 x 250μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865B2 x 50ml
  • Luciferin Detection Reagent

    V859B2 x 1 each
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


2 × 50ml
- G8662 $ 2,320.00 Add to cart

Proteasome-Glo™ Trypsin-Like Cell-Based Assay

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Proteasome-Glo™ Trypsin-Like Cell-Based Assay

 Components

  • Inhibitor 1

    G166A1 x 15μl
  • Inhibitor Mix 2

    G167A1 x 100μl
  • Z-LRR-Glo™ Substrate

    G855A1 x 100μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865A1 x 10ml
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8760 $ 453.00 Add to cart

Proteasome-Glo™ Trypsin-Like Cell-Based Assay

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Proteasome-Glo™ Trypsin-Like Cell-Based Assay

 Components

  • Inhibitor 1

    G166A5 x 15μl
  • Inhibitor Mix 2

    G167A5 x 100μl
  • Z-LRR-Glo™ Substrate

    G855A5 x 100μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865A5 x 10ml
  • Luciferin Detection Reagent

    V859A5 x 1 each
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


5 × 10ml
- G8761 $ 1,418.00 Add to cart

Proteasome-Glo™ Caspase-Like Cell-Based Assay

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Proteasome-Glo™ Caspase-Like Cell-Based Assay

 Components

  • Z-nLPnLD-Glo™ Substrate

    G857A1 x 50μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865A1 x 10ml
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8860 $ 453.00 Add to cart

Proteasome-Glo™ Caspase-Like Cell-Based Assay

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Proteasome-Glo™ Caspase-Like Cell-Based Assay

 Components

  • Z-nLPnLD-Glo™ Substrate

    G857A5 x 50μl
  • Proteasome-Glo™ Cell-Based Buffer

    G865A5 x 10ml
  • Luciferin Detection Reagent

    V859A5 x 1 each
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


5 × 10ml
- G8861 $ 1,418.00 Add to cart

Proteasome-Glo™ 3-Substrate Cell-Based Assay System

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Proteasome-Glo™ 3-Substrate Cell-Based Assay System

 Components

  • Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

    G86601 x 10ml
  • Proteasome-Glo™ Trypsin-Like Cell-Based Assay

    G87601 x 10ml
  • Proteasome-Glo™ Caspase-Like Cell-Based Assay

    G88601 x 10ml
10ml
- G1180 $ 1,126.00 Add to cart

Proteasome-Glo™ 3-Substrate Cell-Based Assay System

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Proteasome-Glo™ 3-Substrate Cell-Based Assay System

 Components

  • Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay

    G86611 x 5 × 10ml
  • Proteasome-Glo™ Trypsin-Like Cell-Based Assay

    G87611 x 5 × 10ml
  • Proteasome-Glo™ Caspase-Like Cell-Based Assay

    G88611 x 5 × 10ml
50ml
- G1200 $ 3,628.00 Add to cart


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Storage Conditions

Store the Proteasome-Glo™ Assay components at –20°C.

For product intended use please see Patents & Disclaimers tab.

The luminogenic substrates containing the Suc-LLVY, Z-LRR or Z-nLPnLD sequence are recognized by the 20S proteasome.Figure 1. The luminogenic substrates containing the Suc-LLVY, Z-LRR or Z-nLPnLD sequence are recognized by the 20S proteasome.
Luminescent signal is proportional to cell number and linear over three logs of cell number; r(2) = 0.99, slope = 0.94.Figure 2. Luminescent signal is proportional to cell number and linear over three logs of cell number (r2 = 0.99, slope = 0.94).
Monitoring proteasome inhibition using different inhibitors and cell lines.Figure 3. Monitoring proteasome inhibition using different inhibitors and cell lines.

Use Restrictions

G8660, G8661, G8662, G8760, G8761, G8860, G8861, G1180, G1200 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8660, G8661, G8662, G8760, G8761, G8860, G8861 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G8660, G8661, G8662, G8760, G8761, G8860, G8861, G1180, G1200 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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