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Promega Corporation

cAMP-Glo™ Max Assay

The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels. The cAMP-Glo™ Max Assay monitors cAMP levels in cells in response to the effect of agonists, antagonists or test compounds on G protein-coupled receptors (GPCRs). The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

cAMP-Glo™ Max Assay

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cAMP-Glo™ Max Assay

 Components

  • Protein Kinase A

    V135A1 x 20μl
  • cAMP-Glo™ ONE Buffer

    V166A1 x 2.5ml
  • MgCl2, 1M

    V330A1 x 1ml
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672A1 x 1 vial
  • Kinase-Glo® Buffer

    V673A1 x 10ml
2 plates
- V1681 $ 328.00 Add to cart

cAMP-Glo™ Max Assay

Close Window

cAMP-Glo™ Max Assay

 Components

  • Protein Kinase A

    V135B1 x 200μl
  • cAMP-Glo™ ONE Buffer

    V166B1 x 20ml
  • MgCl2

    V330B1 x 5ml
  • cAMP, 1mM

    V642A1 x 500μl
  • Kinase-Glo® Substrate

    V672B1 x 1 vial
  • Kinase-Glo® Buffer

    V673B1 x 100ml
20 plates
- V1682 $ 1,833.00 Add to cart

cAMP-Glo™ Max Assay

Close Window

cAMP-Glo™ Max Assay

 Components

  • Protein Kinase A

    V135B10 x 200μl
  • cAMP-Glo™ ONE Buffer

    V166B10 x 20ml
  • MgCl2

    V330B4 x 5ml
  • cAMP, 1mM

    V642A6 x 500μl
  • Kinase-Glo® Substrate

    V672B10 x 1 vial
  • Kinase-Glo® Buffer

    V673B10 x 100ml
10 × 20 plates
- V1683 $ 13,822.00 Add to cart


Storage Conditions

Store the system at –20°C. Before use, completely thaw all components at room temperature, except for the Protein Kinase A, which should be kept on ice when not at –20°C. After thawing, mix all components thoroughly before use. Once prepared, the cAMP detection solution (cAMP-Glo™ ONE Buffer with Protein Kinase A) should not be frozen. Once prepared, the Kinase-Glo® Reagent should be dispensed into aliquots and stored at –20°C. See the product label for the expiration date.

For product intended use please see Patents & Disclaimers tab.

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