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Promega Corporation

cAMP-Glo™ Assay

The cAMP-Glo™ Assay is a homogeneous, bioluminescent and high-throughput assay for measuring cAMP levels in cells. The cAMP-Glo™ Assay monitors cAMP production in cells in response to the effects of test compounds on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

The cAMP-Glo™ Assay can be performed in 96-, 384- or 1536-well ...

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cAMP-Glo™ Assay

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cAMP-Glo™ Assay

 Components

  • Protein Kinase A

    V135D1 x 16μl
  • cAMP-Glo™ Lysis Buffer

    V151A1 x 5ml
  • cAMP-Glo™ Reaction Buffer

    V152A1 x 8ml
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672A1 x 1 vial
  • Kinase-Glo® Buffer

    V673A1 x 10ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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300 assays
(384-well plate)
- V1501 $ 328.00 Add to cart

cAMP-Glo™ Assay

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cAMP-Glo™ Assay

 Components

  • Protein Kinase A

    V135C1 x 160μl
  • cAMP-Glo™ Lysis Buffer

    V151B1 x 25ml
  • cAMP-Glo™ Reaction Buffer

    V152B1 x 50ml
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672B1 x 1 vial
  • Kinase-Glo® Buffer

    V673B1 x 100ml
3,000 assays
(384-well plate)
- V1502 $ 1,833.00 Add to cart

cAMP-Glo™ Assay

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cAMP-Glo™ Assay

 Components

  • Protein Kinase A

    V135C10 x 160μl
  • cAMP-Glo™ Lysis Buffer

    V151C1 x 250ml
  • cAMP-Glo™ Reaction Buffer

    V152C1 x 500ml
  • cAMP, 1mM

    V642A1 x 500μl
  • Kinase-Glo® Substrate

    V672B10 x 1 vial
  • Kinase-Glo® Buffer

    V673B10 x 100ml
30,000 assays
(384-well plate)
- V1503 $ 13,822.00 Add to cart


Storage Conditions

Store the system at –20°C. Once prepared, the cAMP detection solution (cAMP-Glo™ Reaction Buffer with Protein Kinase A) should not be frozen. Once prepared, the Kinase-Glo® Reagent should be dispensed into aliquots and stored at –20°C. See the product label for the expiration date.

For product intended use please see Patents & Disclaimers tab.

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