Maximize Recovery of Nucleic Acid from FFPE Tissues with Novel Flexible Purification Technology

Part # PS262

Abstract

Doug Wieczorek PhD, Brad Hook PhD, Eric Vincent PhD, and Trista Schagat PhD
Promega Corporation, 2800 Woods Hollow, Madison WI, USA 53711

The ReliaPrep™ FFPE gDNA Miniprep System uses a novel purification technology that does not rely on use of any harsh organic solvents and concentrates the NA for low volume elution on a specially designed spin column, thus maximizing concentration. Using this technology, we examined several aspects of the purification protocol for flexibility and adaptability: effect of decrosslinking time on the quality and length of nucleic acids released from diverse FFPE tissues, effect of overnight storage between lysis and extraction steps, and whether the technology could be adapted to extract both DNA and RNA from the same FFPE tissue sample.

Printed in USA.