Promega Corporation

RNasin® Ribonuclease Inhibitors

Natural and Recombinant RNasin® Ribonuclease Inhibitors have broad-spectrum RNase inhibitory properties, including the inhibition of eukaryotic RNases of the neutral type. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. The Ki value for the binding of RNasin® Ribonuclease Inhibitor to RNase (e.g., RNase A) is approximately 10–14M. Promega offers two different preparations: Recombinant RNasin® Ribonuclease Inhibitor and Natural RNasin® Ribonuclease Inhibitor. Both products are purified using a combination of ion exchange and affinity chromatography.

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Recombinant RNasin® Ribonuclease Inhibitor

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Recombinant RNasin® Ribonuclease Inhibitor

 Components

  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
2,500u
20–40u/μl N2511 $ 107.00 Add to cart

Recombinant RNasin® Ribonuclease Inhibitor

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Recombinant RNasin® Ribonuclease Inhibitor

 Components

  • Recombinant RNasin® Ribonuclease Inhibitor

    N251B1 x 10,000u
10,000u
20–40u/μl N2515 $ 261.00 Add to cart


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QC Tests

SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.

Source

Recombinant strain of E. coli (Cat.# N2511, N2515) or human placenta (Cat.# N2111, N2115).

Storage Buffer

20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

N2511, N2515 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

Cat.# N2511, N2515: U.S. Pat. No. 5,552,302.

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