ReliaPrep FFPE DNA and RNA Miniprep Systems

Fixate on the possibilities

ReliaPrep™ FFPE Miniprep Systems provide complete, all-inclusive methods for purification of gDNA and total RNA from formalin-fixed paraffin-embedded tissues.

  • Isolate quality, intact nucleic acids: Optimized chemistry reverses modifications introduced by the fixation process, resulting in intact, amplifiable nucleic acids.
  • Easy to use: Minimal preparation time; simply add ethanol and go!
  • Safely deparaffinize your sample: Deparaffinize without using harsh organic solvents.
  • Single FFPE Sample: Purify both DNA and RNA from a single FFPE tissue sample.
  • Save time: Purify gDNA and total RNA from FFPE tissue in less than two and one half hours with minimal hands on time. No overnight digestion required.

DNA isolated using the ReliaPrep™ FFPE Kit Provides Superior Performance Compared to Competitor in qPCR Analysis

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Genomic DNA purified from a single 10µm mouse brain section using either the ReliaPrep™ FFPE gDNA Miniprep System or competitor kit. Samples were analyzed using GoTaq® qPCR System. Earlier Cq values were obtained with the ReliaPrep™ FFPE Miniprep System.

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Genomic DNA purified from a single 10µm mouse brain section using either the ReliaPrep™ FFPE gDNA Miniprep System or competitor kit. Samples were analyzed using GoTaq® qPCR System. Earlier Cq values were obtained with the ReliaPrep™ FFPE Miniprep System.

Multiplex PCR of variable length, end-point PCR fragments of GAPDH gene from nucleic acid purified from 10µm slices of FFPE human liver.

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Panel A. 10µl purified gDNA used as template in multiplex PCR (GoTaq® Hot Start). Fragments ranging from 100-400bp detected.

Panel B. 5µl purified RNA used as template in GoScript™ Reverse Transcription System. 5µl of cDNA product used as template for the multiplex PCR (GoTaq® Hot Start). Fragments from 80bp-290bp detected.

All reactions performed in triplicate from sequential slices. 10µl PCR product run on a 2% agarose gel.

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Panel A. 10µl purified gDNA used as template in multiplex PCR (GoTaq® Hot Start). Fragments ranging from 100-400bp detected.

Panel B. 5µl purified RNA used as template in GoScript™ Reverse Transcription System. 5µl of cDNA product used as template for the multiplex PCR (GoTaq® Hot Start). Fragments from 80bp-290bp detected.

All reactions performed in triplicate from sequential slices. 10µl PCR product run on a 2% agarose gel.

STR Analysis of DNA Isolated from FFPE Liver Tissue Using the ReliaPrep™ FFPE Kit.

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Genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System. STR analysis was performed using Promega's Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641). This system is compatible with STR amplification and MSI detection.

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Genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System. STR analysis was performed using Promega's Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641). This system is compatible with STR amplification and MSI detection.

Customer Data Generated Using ReliaPrep™ FFPE DNA and RNA Minipreps

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ReliaPrep™ FFPE gDNA Miniprep Sample Type: Human cervical cancer, 3 years old. Description: Genomic DNA was extracted using Qiagen (lane 1) and Promega ReliaPrep FFPE kits (lanes 2-5). Lane M = DNA marker, Lane N = blank.