The QuantiFluor® RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is very important step that is used in many biological applications, particularly in molecular biology techniques. These include measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PC.
Sensitivity: Significantly increased sensitivity over absorbance at 260nm (NanoDrop) for those samples that are low in concentration. The QuantiFluor RNA System is designed to measure 0.1–500ng/µl of RNA (from 1µl sample in a 200µl assay).
Less Template RNA Required: Other methods require much more RNA (~1 µg) to accurately quantitate RNA, compared to QuantiFluor® RNA Dye (only 100 pg). Save more of your precious sample for downstream assays.
Ease of Use: System includes all the necessary reagents to quickly set up and quantitate RNA.
Offers an integrated solution with instrument pairing. Sets up easily on microplate or single tube fluorometers from Promega.
Cat #E6105 Quantus™ Fluorometer
Using Blue Channel (Ex 460 nm, Em 515 - 575 nm)
Cat.# GM3000 GloMax® Discover
Cat.# GM3500 Glomax® Explorer
Cat #E6070 or #E6080 GloMax®-Multi Jr
With Cat #E6071 Fluorescence Optical Kit - BLUE (Ex 460 nm, Em 515 - 570 nm)
For research use only. Not for use in diagnostic procedures. QuantiFluor is a trademark of Promega Corporation. All other trademarks are the sole property of their respective owners.