Promega Corporation

QuantiFluor™ RNA System

The QuantiFluor™ RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is a very important step used in many biological applications, particularly in molecular biology techniques. These include measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation and reverse transcription PCR.

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QuantiFluor™ RNA System
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QuantiFluor™ RNA System  Components

  • 20X TE Buffer (pH 7.5)

    E260A1 x 25ml
  • RNA Standard

    E299A1 x 100μg
  • QuantiFluor™ RNA Dye

    E286A1 x 1ml
1ml
- E3310 $ 260.00 Add to cart


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Storage Conditions

Store at –30°C to –10°C, protected from light.

For product intended use please see Patents & Disclaimers tab.

Figures

10402MA_500pxFigure 1. Standard curves using the QuantiFluor™ RNA Dye and a competitor dye.

The standard curves were generated using RNA Standard in a 96-well format and 200µl total volume as described in Section 5 of the Technical Manual. The standard curve RNA amounts are 2ng, 10ng, 20ng, 50ng, 100ng, 200ng and 500ng per well. Fluorescence was measured using the GloMax®-Multi+ Detection System. The fluorescence values shown were blank-subtracted. Under these conditions, the dynamic range for the QuantiFluor™ RNA Dye is approximately 2–500ng per well (in 200µl total volume), and the QuantiFluor™ RNA Dye limit of detection is approximately 100pg per well.

Use Restrictions

E3310 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

Patent Pending.
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