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Promega Corporation

TSAP Thermosensitive Alkaline Phosphatase

TSAP Thermosensitive Alkaline Phosphatase catalyzes the removal of 5´ phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3´ overhangs, 5´ overhangs and blunt ends. It is also useful for preparing DNA for 5´ end-labeling by removing existing phosphate groups from the 5´ end.

TSAP is irreversibly inactivated by heating at 74°C for 15 minutes. Therefore, a DNA cleanup step is not required before proceeding to a ligation reaction. TSAP is fully active in all restriction enzyme reaction buffers tested under the conditio...

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TSAP Thermosensitive Alkaline Phosphatase

100 units
- M9910 $ 66.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, endonuclease, DNase, RNase, blue/white cloning assay.

Source

Bacteria.

Storage Buffer

10mM Tris (pH 7.5 @ 25°C), 100mM NaCl, 50% glycerol.

Storage Conditions

Store at –20°C. See the expiration date on the label.

Unit Definition

One MBU is defined as the amount of enzyme required to dephosphorylate 1μg of pGEM® DNA with 3´ overhangs in 15 minutes at 37°C in a 50μl reaction using any Promega restriction enzyme buffer (except F). Dephosphorylation is defined as preventing >95% of the plasmid from ligating (analyzed by agarose gel electrophoresis).

For product intended use please see Patents & Disclaimers tab.

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