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pSP64 Poly(A) Vector

The pSP64 Poly(A) Vector can be used as a standard cloning vector and for in vitro transcription from the SP6 promoter. The pSP64 Poly(A) Vector also can be used to generate poly(A)+ transcripts in vitro. The vector has a stretch of 30 dA:dT residues inserted between the SacI and EcoRI sites. Therefore, when foreign DNA is cloned into any polylinker site other than EcoRI (HindIII, PstI, SalI, AccI, HincII, XbaI, BamHI, AvaI, SmaI or SacI), linearization of the recombinant plasmid with EcoRI allows the use of SP6 RNA polymerase in vitro to prepare RNA copies of the inserted sequences that contain a synthetic 3´ "po...

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pSP64 Poly(A) Vector

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pSP64 Poly(A) Vector

 Components

  • pSP64 Poly(A) Vector

    P124A1 x 20μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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20μg
- P1241 $ 96.00 Add to cart


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GenBank®/EMBL Accession Number

pSP64 Poly(A) Vector Accession Number X65328; pSP64 Poly(A) Vector sequence text file.
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Storage Conditions

Store vector at –20°C.

For product intended use please see Patents & Disclaimers tab.

pSP64 Poly(A) Vector.Figure 1. pSP64 Poly(A) Vector.

Use Restrictions

P1241 For Research Use Only. Not for Use in Diagnostic Procedures.

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