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Promega Corporation

Mung Bean Nuclease

Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends. Mung Bean Nuclease has been used for transcript mapping studies, for flushing staggered ends and for the separation of cDNA strands after synthesis with reverse transcriptase and DNA Polymerase I.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Mung Bean Nuclease

2,000u
50–100u/μl M4311 $ 92.00 Add to cart


QC Tests

Activity, endonuclease.

Source

Mung bean sprouts.

Storage Buffer

10mM Tris-HCl (pH 7.5 at 25°C), 50mM NaCl, 0.01% Triton® X-100 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble nucleotides per minute at 37°C in 30mM sodium acetate (pH 5.0), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.

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