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Promega Corporation

HaloTag® Vectors for E. coli and Cell-Free Protein Expression

The following vectors are used for inducible expression of HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein (e.g., pFN18A/K, Cat.# G2751, G2681) can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied in two formats: as multiple cloning site (MCS) vectors for traditional cloning and as Flexi® System vectors.

The Flexi® Vector System is a simple, directional cloning met...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart
pH6HTN His6HaloTag® T7 Vector
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pH6HTN His6HaloTag® T7 Vector  Components

  • pH6HTN His6HaloTag® T7 Vector

    G797A1 x 20μg
20μg
- G7971 $ 205.00 Add to cart
pH6HTC His6HaloTag® T7 Vector
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pH6HTC His6HaloTag® T7 Vector  Components

  • pH6HTC His6HaloTag® T7 Vector

    G803A1 x 20μg
20μg
- G8031 $ 205.00 Add to cart

pF1A T7 Flexi® Vector

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pF1A T7 Flexi® Vector

 Components

  • pF1A T7 Flexi® Vector

    C844A1 x 20μg
20μg
- C8441 $ 263.00 Add to cart

pF1K T7 Flexi® Vector

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pF1K T7 Flexi® Vector

 Components

  • pF1K T7 Flexi® Vector

    C845A1 x 20μg
20μg
- C8451 $ 263.00 Add to cart

pFN18A HaloTag® T7 Flexi® Vector

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pFN18A HaloTag® T7 Flexi® Vector

 Components

  • pFN18A HaloTag® T7 Flexi® Vector

    G275A1 x 20μg
20μg
- G2751 $ 224.00 Add to cart

pFN18K HaloTag® T7 Flexi® Vector

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pFN18K HaloTag® T7 Flexi® Vector

 Components

  • pFN18K HaloTag® T7 Flexi® Vector

    G268A1 x 20μg
20μg
- G2681 $ 224.00 Add to cart

pFN19A HaloTag® T7 SP6 Flexi® Vector

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pFN19A HaloTag® T7 SP6 Flexi® Vector

 Components

  • pFN19A HaloTag® T7 SP6 Flexi® Vector

    G189A1 x 20μg
20μg
- G1891 $ 224.00 Add to cart

pFN19K HaloTag® T7 SP6 Flexi® Vector

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pFN19K HaloTag® T7 SP6 Flexi® Vector

 Components

  • pFN19K HaloTag® T7 SP6 Flexi® Vector

    G184A1 x 20μg
20μg
- G1841 $ 224.00 Add to cart

pFC20A HaloTag® T7 SP6 Flexi® Vector

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pFC20A HaloTag® T7 SP6 Flexi® Vector

 Components

  • pFC20A HaloTag® T7 SP6 Flexi® Vector

    G168A1 x 20μg
20μg
- G1681 $ 224.00 Add to cart

pFC20K HaloTag® T7 SP6 Flexi® Vector

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pFC20K HaloTag® T7 SP6 Flexi® Vector

 Components

  • pFC20K HaloTag® T7 SP6 Flexi® Vector

    G169A1 x 20μg
20μg
- G1691 $ 224.00 Add to cart
pFN29A His6HaloTag® T7 Flexi® Vector
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pFN29A His6HaloTag® T7 Flexi® Vector  Components

  • pFN29A His6HaloTag® T7 Flexi® Vector

    G826A1 x 20μg
20μg
- G8261 $ 224.00 Add to cart
pFN29K His6HaloTag® T7 Flexi® Vector
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pFN29K His6HaloTag® T7 Flexi® Vector  Components

  • pFN29K His6HaloTag® T7 Flexi® Vector

    G833A1 x 20μg
20μg
- G8331 $ 224.00 Add to cart
pFC30A His6HaloTag® T7 Flexi® Vector
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pFC30A His6HaloTag® T7 Flexi® Vector  Components

  • pFC30A His6HaloTag® T7 Flexi® Vector

    G832A1 x 20μg
20μg
- G8321 $ 224.00 Add to cart
pFC30K His6HaloTag® T7 Flexi® Vector
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pFC30K His6HaloTag® T7 Flexi® Vector  Components

  • pFC30K His6HaloTag® T7 Flexi® Vector

    G838A1 x 20μg
20μg
- G8381 $ 224.00 Add to cart


You Also May Be Interested In

GenBank®/EMBL Accession Number

pH6HTC His6HaloTag® T7 Accession Number JN874647; pH6HTC His6HaloTag® T7 Vector sequence text file.
pH6HTN His6HaloTag® T7 Accession Number JN874646; pH6HTN His6HaloTag® T7 Vector sequence text file.
pFN29A His6HaloTag® T7 Accession Number JN874648; pFN29A His6HaloTag® T7 Vector sequence text file.
pFN29K His6HaloTag® T7 Accession Number JN874650; pFN29K His6HaloTag® T7 Vector sequence text file.
pFC30A His6HaloTag® T7 Accession Number JN874649; pFC30A His6HaloTag® T7 Vector sequence text file.
pFC30K His6HaloTag® T7 Accession Number JN874651; pFC30K His6HaloTag® T7 Vector sequence text file.
pF1A T7 Accession Number AY753576; pF1A T7 Vector sequence text file.
pF1K T7 Accession Number AY753577; pF1K T7 Vector sequence text file.
pFN18A HaloTag® T7 Accession Number EU545992; pFN18A HaloTag® T7 Vector sequence text file.
pFN18K HaloTag® T7 Accession Number EU545993; pFN18K HaloTag® T7 Vector sequence text file.
pFN19A HaloTag® T7 SP6 Accession Number EU545994; pFN19A HaloTag® T7 SP6 Vector sequence text file.
pFN19K HaloTag® T7 SP6 Accession Number EU545995; pFN19K HaloTag® T7 SP6 Vector sequence text file.
pFC20A HaloTag® T7 SP6 Accession Number EU545996; pFC20A HaloTag® T7 SP6 Vector sequence text file.
pFC20K HaloTag® T7 SP6 Accession Number EU545997; pFC20K HaloTag® T7 SP6 Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

HaloTag® Technology improves purification of difficult proteins.Figure 1. HaloTag® Technology improves purification of difficult proteins.

Covalent, irreversible binding of HaloTag® fusion tag to the HaloLink™ Resin maximizes purification yields when compared to other fusion tags.

Covalent, irreversible binding of HaloTag® fusion tag to the HaloLink™ Resin maximizes purification yields when compared to other fusion tags.

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pH6HTN His6HaloTag® T7 Vector.Figure 2. pH6HTN His6HaloTag® T7 Vector.
pH6HTC His6HaloTag® T7 Vector.Figure 3. pH6HTC His6HaloTag® T7 Vector.
pF1A T7 Flexi Vector.Figure 4. pF1A T7 Flexi® Vector.
pF1K T7 Flexi Vector.Figure 5. pF1K T7 Flexi® Vector.
pFN18A HaloTag T7 Flexi Vector 7496MAFigure 6. pFN18A HaloTag® T7 Flexi® Vector.
pFN18K HaloTag T7 Flexi Vector 7497MAFigure 7. pFN18K HaloTag® T7 Flexi® Vector.
pFN19A HaloTag T7 SP6 Flexi Vector 7498MAFigure 8. pFN19A HaloTag® T7 SP6 Flexi® Vector.
PFN19K HaloTag T7 SP6 Flexi Vector 7499MAFigure 9. pFN19K HaloTag® T7 SP6 Flexi® Vector.
pFC20A HaloTag T7 SP6 Flexi Vector. Figure 10. pFC20A HaloTag® T7 SP6 Flexi® Vector.
pFC20K HaloTag T7 SP6 Flexi Vector.Figure 11. pFC20K HaloTag® T7 SP6 Flexi® Vector.
pFN29A His6HaloTag® T7 Flexi® Vector.Figure 12. pFN29A His6HaloTag® T7 Flexi® Vector.
pFN29K His6HaloTag® T7 Flexi® Vector.Figure 13. pFN29K His6HaloTag® T7 Flexi® Vector.
pFC30A His6HaloTag® T7 Flexi® Vector.Figure 14. pFC30A His6HaloTag® T7 Flexi® Vector.
pFC30K His6HaloTag® T7 Flexi® Vector.Figure 15. pFC30K His6HaloTag® T7 Flexi® Vector.
HaloTag Flexi Vectors.Table 1. HaloTag® Flexi® Vectors.

Use Restrictions

G7971, G8031, C8441, C8451, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G7971, G8031, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G7971, G8031, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 U.S. Pat. Nos. 7,425,436 and 7,935,803 and other patents pending.

C8441, C8451, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 European Pat. No. 1685247 and other patents pending.

C8441, C8451 Patent Pending.

G7971, G8031, C8441, C8451, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 Usage Restrictions for the T7 Expression System
The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused.

Academic and NonProfit Laboratories
No materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.

Commercial Laboratories
A license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, FAX: 631-344-3729.

G7971, G8031, C8441, C8451, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.

C8441, C8451, G2751, G2681, G1891, G1841, G1681, G1691, G8261, G8331, G8321, G8381 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

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