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Promega Corporation

HaloTag® Vectors for E. coli and Cell-Free Protein Expression

The following vectors are used for inducible expression of HaloTag® fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag® fusion protein (e.g., pFN18A/K, Cat.# G2751, G2681) can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag® vectors are supplied in two formats: as multiple cloning site (MCS) vectors for traditional cloning and as Flexi® System vectors.

The Flexi® Vector System is a simple, directional cloning met...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart
pH6HTN His6HaloTag® T7 Vector 20μg
- G7971 $ 205.00 Add to cart
pH6HTC His6HaloTag® T7 Vector 20μg
- G8031 $ 205.00 Add to cart

pF1A T7 Flexi® Vector

20μg
- C8441 $ 263.00 Add to cart

pF1K T7 Flexi® Vector

20μg
- C8451 $ 263.00 Add to cart

pFN18A HaloTag® T7 Flexi® Vector

20μg
- G2751 $ 224.00 Add to cart

pFN18K HaloTag® T7 Flexi® Vector

20μg
- G2681 $ 224.00 Add to cart

pFN19A HaloTag® T7 SP6 Flexi® Vector

20μg
- G1891 $ 224.00 Add to cart

pFN19K HaloTag® T7 SP6 Flexi® Vector

20μg
- G1841 $ 224.00 Add to cart

pFC20A HaloTag® T7 SP6 Flexi® Vector

20μg
- G1681 $ 224.00 Add to cart

pFC20K HaloTag® T7 SP6 Flexi® Vector

20μg
- G1691 $ 224.00 Add to cart
pFN29A His6HaloTag® T7 Flexi® Vector 20μg
- G8261 $ 224.00 Add to cart
pFN29K His6HaloTag® T7 Flexi® Vector 20μg
- G8331 $ 224.00 Add to cart
pFC30A His6HaloTag® T7 Flexi® Vector 20μg
- G8321 $ 224.00 Add to cart
pFC30K His6HaloTag® T7 Flexi® Vector 20μg
- G8381 $ 224.00 Add to cart


GenBank®/EMBL Accession Number

pH6HTC His6HaloTag® T7 Accession Number JN874647; pH6HTC His6HaloTag® T7 Vector sequence text file.
pH6HTN His6HaloTag® T7 Accession Number JN874646; pH6HTN His6HaloTag® T7 Vector sequence text file.
pFN29A His6HaloTag® T7 Accession Number JN874648; pFN29A His6HaloTag® T7 Vector sequence text file.
pFN29K His6HaloTag® T7 Accession Number JN874650; pFN29K His6HaloTag® T7 Vector sequence text file.
pFC30A His6HaloTag® T7 Accession Number JN874649; pFC30A His6HaloTag® T7 Vector sequence text file.
pFC30K His6HaloTag® T7 Accession Number JN874651; pFC30K His6HaloTag® T7 Vector sequence text file.
pF1A T7 Accession Number AY753576; pF1A T7 Vector sequence text file.
pF1K T7 Accession Number AY753577; pF1K T7 Vector sequence text file.
pFN18A HaloTag® T7 Accession Number EU545992; pFN18A HaloTag® T7 Vector sequence text file.
pFN18K HaloTag® T7 Accession Number EU545993; pFN18K HaloTag® T7 Vector sequence text file.
pFN19A HaloTag® T7 SP6 Accession Number EU545994; pFN19A HaloTag® T7 SP6 Vector sequence text file.
pFN19K HaloTag® T7 SP6 Accession Number EU545995; pFN19K HaloTag® T7 SP6 Vector sequence text file.
pFC20A HaloTag® T7 SP6 Accession Number EU545996; pFC20A HaloTag® T7 SP6 Vector sequence text file.
pFC20K HaloTag® T7 SP6 Accession Number EU545997; pFC20K HaloTag® T7 SP6 Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

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