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Promega Corporation

DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus

DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and the 3´→5´ exonuclease activities present in intact E. coli DNA Polymerase I. It is used for random primer labeling and in strand displacement amplification. Klenow Fragment, Exonuclease Minus, will leave a single-base 3´ overhang on a significant proportion of DNA fragments during fill-in of 5´-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation.

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Klenow Fragment, Exonuclease Minus

100u
5–10u/μl M2181 $ 55.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, endonuclease, DNase, RNase.

Source

Recombinant E. coli strain.

Storage Buffer

50mM Tris-HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmol of total deoxyribonucleotides into TCA-insoluble material in 30 minutes at 37°C. The reaction conditions are: 67mM potassium phosphate (pH 7.4 at 25°C), 6.7mM MgCl2, 1mM DTT, 50μg/ml activated calf thymus DNA, and 33μM dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).

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